TY - JOUR 
A1 - Penyalver, Ramón
AU - Roesch, Luis F. W.
AU - Piquer-Salcedo, Jaime E.
AU - Forner-Giner, María A.
AU - Alguacil, María M.
T1 - From the bacterial citrus microbiome to the selection of potentially host-beneficial microbes
Y1 - 2022
SN - 1871-6784
UR - http://hdl.handle.net/20.500.11939/8245
AB - Citrus is the most cultivated fruit crop worldwide. The modern citrus industry needs new bioproducts to overcome phytopathological threats, tolerate stresses and increase yield and quality. Mutualistic microbes from roots significantly impact host physiology and health and are a potentially beneficial resource. The bacterial microbiome can be surveyed to select potentially host-beneficial microbes. To achieve this goal, a prevalent “core-citrus” bacterial microbiome was obtained by picking those operational taxonomic units (OTUs) shared among samples within and across two Citrus rootstock genotypes grown in the same soil for more than 20 years. A sub-selection of main OTUs from the defined "core-citrus" microbiome was made based on abundance, host-enriched versus bulk soil, and rhizosphere-indicator species. In parallel, an extensive census of the cultivable microbiota was performed to collect a large number of bacterial citrus isolates. Metataxonomic data were linked to cultured microbes, matching 16S rRNA gene sequences from bacterial isolates with those counterpart OTU reference sequences from the selected bacterial "core-citrus" microbiome. This approach allowed selection of potentially host-beneficial bacteria to mine for agricultural probiotics in future biotechnological applications required for the citrus industry.
KW - Citrus microbiomes
KW - Metataxonomics
KW - Culturomics
KW - Rhizospheric bacteria
KW - Beneficial bacteria
KW - Agricultural probiotics
KW - H20  Plant diseases
KW - U40  Surveying methods
KW - F30  Plant genetics and breeding
KW - A50  Agricultural research
KW - P34  Soil biology
LA - en
PB - Elsevier
ER -