TY - JOUR 
A1 - Lloret, Alba
AU - Martínez-Fuentes, Amparo
AU - Agustí, Manuel
AU - Badenes, María L.
AU - Ríos, Gabino
T1 - Chromatin-associated regulation of sorbitol synthesis in flower buds of peach
Y1 - 2017
SN - 0167-4412
UR - http://hdl.handle.net/20.500.11939/5736
AB - Key message PpeS6PDH gene is postulated to mediate
sorbitol synthesis in flower buds of peach concomitantly
with specific chromatin modifications.
Abstract Perennial plants have evolved an adaptive mechanism
involving protection of meristems within specialized
structures named buds in order to survive low temperatures
and water deprivation during winter. A seasonal period of
dormancy further improves tolerance of buds to environmental
stresses through specific mechanisms poorly known at the
molecular level. We have shown that peach PpeS6PDH gene
is down-regulated in flower buds after dormancy release,
concomitantly with changes in the methylation level at specific
lysine residues of histone H3 (H3K27 and H3K4) in
the chromatin around the translation start site of the gene.
PpeS6PDH encodes a NADPH-dependent sorbitol-6-phosphate
dehydrogenase, the key enzyme for biosynthesis of
sorbitol. Consistently, sorbitol accumulates in dormant
buds showing higher PpeS6PDH expression. Moreover,
PpeS6PDH gene expression is affected by cold and water
deficit stress. Particularly, its expression is up-regulated by
low temperature in buds and leaves, whereas desiccation
treatment induces PpeS6PDH in buds and represses the gene in leaves. These data reveal the concurrent participation of
chromatin modification mechanisms, transcriptional regulation
of PpeS6PDH and sorbitol accumulation in flower buds
of peach. In addition to its role as a major translocatable
photosynthate in Rosaceae species, sorbitol is a widespread
compatible solute and cryoprotectant, which suggests its
participation in tolerance to environmental stresses in flower
buds of peach.
KW - Abiotic stress
KW - Bud dormancy
KW - Chromatin
KW - Prunus persica
KW - Sorbitol-6-phosphate dehydrogenase (S6PDH)
KW - Aldose-6-phosphate reductase (Ald6PRase)
KW - F30  Plant genetics and breeding
KW - Prunus persica
KW - Bud dormancy
KW - Abiotic stress
KW - Chromatin
LA - en
PB - Springer
ER -