TY - JOUR 
A1 - Galipienso, Luis
AU - Rubio, Luis
AU - Lopez, Carmelo
AU - Soler, Salvador
AU - Aramburu, Jose
T1 - Complete nucleotide sequence of a Spanish isolate of Parietaria mottle virus infecting tomato
Y1 - 2009
SN - 0920-8569
UR - http://hdl.handle.net/20.500.11939/5228
AB - The genome of a Spanish isolate of Parietaria mottle virus (PMoV) obtained from tomato (strain PMoV-T) was completely sequenced. Protein motifs conserved for RNA viruses were identified: the p1 protein contained a metyltransferase domain in its N-terminal half and a triphosphatase/helicase domain in its C-terminal half, the p2 protein contained a RNA polymerase domain; the 3a protein contained a RNA-binding domain with alpha-helix and beta-sheet secondary structures. In addition, stem-loop structures with potential capacity of protein interactions were predicted on the untranslated terminal regions. Comparison with the other sequenced PMoV isolate showed nucleotide identities of 93, 90, and 93% for genomic RNAs 1, 2 and 3, respectively, and amino acid identities ranging from 88 to 97% for the different proteins. A cytosine deletion was detected at position 1,366 of RNA 3, involving a start codon for the coat protein (CP) gene different from the other PMoV isolate, resulting in a CP 16 amino acids shorter. Comparison of synonymous and nonsynonymous mutations revealed different selective constraints along the genome.
LA - en
ER -