TY - JOUR 
A1 - Olivares-Fuster, Oscar
AU - Pena, Leandro
AU - Durán-Vila, Núria
AU - Navarro, Luis
T1 - Green fluorescent protein as a visual marker in somatic hybridization
Y1 - 2002
SN - 0305-7364
UR - http://hdl.handle.net/20.500.11939/4229
AB - Using a transgenic citrus plant expressing Green Fluorescent Protein (GFP) as a parent in somatic fusion experiments, we investigated the suitability of GFP as an in vivo marker to follow the processes of protoplast fusion, regeneration and selection of hybrid plants. A high level of GFP expression was detected in transgenic citrus protoplasts, hybrid callus, embryos and plants, It is demonstrated that GFP can be used for the continuous monitoring of the fusion process, localization of hybrid colonies and callus, and selection of somatic hybrid embryos and plants.
LA - en
ER -