TY - JOUR 
A1 - Novillo, Pedro
AU - Salvador, Alejandra
AU - Magalhaes, Tahise
AU - Besada, Cristina
T1 - Deastringency treatment with CO2 induces oxidative stress in persimmon fruit
Y1 - 2014
SN - 0925-5214
UR - http://hdl.handle.net/20.500.11939/4222
AB - Because of astringency at harvest, 'Rojo Brillante' persimmons are regularly submitted to deastringency treatment based on exposing fruit to a high CO2 concentration. The treatment conditions that ensure total astringency removal throughout the various maturity stages have been determined to be 95% CO2, 20 degrees C, 24 h. The aim of this study was to investigate changes in the redox state of persimmon fruit associated with this deastringency treatment. The levels of reactive oxygen species (ROS) (O-2(-) and H2O2), and the activities of the main ROS scavenging enzymes (CAT, POD, APX, and SOD), were determined at harvest and after deastringency in fruit at three different maturity stages. Our results showed that during 'Rojo Brillante' persimmon maturation, the level of O-2(-) gradually increased, while APX activity was lowered. The deastringency treatment with CO2 induced oxidative stress in the fruit, observed as an over-accumulation of O-2(-) and H2O2. As a response to ROS accumulation, the activities of the CAT, APX and SOD scavenging enzymes were up-regulated after deastringency treatment. The response of POD enzyme was dependent on maturity stage, showing enhanced activity after CO2 treatment only for the fruit at the most mature stage. (C) 2014 Elsevier B.V. All rights reserved.
LA - en
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