RT conferenceObject
T1 Estimation of the Number of Citrus tristeza virus-Viruliferous Aphids Landing on Individual Citrus Seedlings and Viral Incidence in Different Citrus Rootstocks in Spain
A1 Rodriguez, A.
A1 Gorris, María T.
A1 Serra, J.
A1 Roman, M. P.
A1 Collado, C.
A1 Gimenez, P.
A1 Bertolini, Edson
A1 Hermoso-De-Mendoza, Alfonso
A1 Cambra, Mariano
K1 Carrizo citrange
K1 H10  Pests of plants
K1 H20  Plant diseases
K1 U30  Research methods
K1 Citrus tristeza virus
K1 Aphids
K1 Aphis gossypii
K1 Myzus persicae
K1 Aphis spiraecola
K1 Sour orange
K1 Resistance to disease
K1 Rootstocks
K1 Vectors
AB The number of aphid species landing, from January to June 2004, on alemow, Cleopatra mandarin and Carrizo citrange grown in experimental nursery blocks in the same area in the north of the Valencian Community, were estimated by counting the number of young shoots/plant and the number of aphids trapped on sticky shoots. The proportions of different aphid species represented by captured individuals (10,569) were: Aphis gossypii (34.9%), Myzus persicae (12.7%), A. spiraecola (8.6%), A. craccivora (2.4%), A. fabae (0.8%), Toxoptera aurantii (0.8%) and others (39.9%). C. macrophylla was the most visited citrus species with estimated 1,656 aphids landing/plant during the test period. May was the month with higher aphid populations. The number and percentage of aphids carrying Citrus tristeza virus (CTV) was determined by a validated nested-PCR method in a single closed tube using extracted RNA from previously trapped aphids that were squashed on paper. Viral RNA was detected in 13.3% of tested A. gossypii individuals. CTV infection rates assessed by tissue print-ELISA in experimental plots located in the same area were proportional to the number of aphids landing on each susceptible host. Average annual CTV infection incidence ranged from approximately 4% for alemow and Volkamer lemon to 2% in Cleopatra mandarins. Nevertheless, despite the number of CTV viruliferous vectors visiting Carrizo citrange, sour orange and citrumelo, CTV incidence was only 0.6%, 0.2% and 0%, respectively, suggesting some resistance to natural (aphid-vectored) infection in these particular citrus seedlings.
PB IOCV
SN 2313-5123
YR 2005
FD 2005
LK http://hdl.handle.net/20.500.11939/7818
UL http://hdl.handle.net/20.500.11939/7818
LA es
NO Rodríguez, A., Gorris, M. T., Serra, J., Román, M. P., Collado, C., Hermoso-de-Mendoza, A. et al. (2005). Estimation of the number of Citrus tristeza virus-viruliferous aphids landing on individual citrus seedlings and viral incidence in different citrus rootstocks in Spain. Proceedings of the Sixteenth Conference of the International Organization of Citrus Virologists, 399-403.
DS MINDS@UW
RD Aug 18, 2022