RT article
T1 Role of the yeast multidrug transporter Qdr2 in cation homeostasis and the oxidative stress response
A1 Ríos, Gabino
A1 Cabedo-López, Marc
A1 Rull, Baltasar
A1 Yenush, Lynne
A1 Serrano, Ramón
A1 Mulet, José M.
K1 Ion transport
K1 Copper homeostasis
K1 F62  Plant physiology - Growth and development
K1 Oxidative stress
AB We have identified QDR2 in a screening for genes able to confer tolerance to sodium and/or lithium stress upon overexpression. Qdr2 is a multidrug transporter of the major facilitator superfamily, originally described for its ability to transport the antimalarial drug quinidine and the herbicide barban. To identify its physiological substrate, we have screened for phenotypes dependent on QDR2 and found that Qdr2 is able to transport monovalent and divalent cations with poor selectivity, as shown by growth tests and the determination of internal cation content. Moreover, strains overexpressing or lacking QDR2 also exhibit phenotypes when reactive oxygen species- producing agents, such as hydrogen peroxide or menadione were added to the growth medium. We have also found that the presence of copper and hydrogen peroxide repress the expression of QDR2. In addition, the copper uptake of a qdr2 mutant strain is similar to a wild type, but the extrusion is clearly impaired. Based on our results, we propose that free divalent copper is the main physiological substrate of Qdr2. As copper is a substrate for several redox reactions that occur within the cytoplasm, its function in copper homeostasis explains its role in the oxidative stress response.
PB Oxford University Press
SN 1567-1364
YR 2013
FD 2013
LK http://hdl.handle.net/20.500.11939/6495
UL http://hdl.handle.net/20.500.11939/6495
LA en
NO Ríos, G., Cabedo, M., Rull, B., Yenush, L., Serrano, R., & Mulet, J. M. (2013). Role of the yeast multidrug transporter Qdr2 in cation homeostasis and the oxidative stress response. FEMS yeast research, 13(1), 97-106.
DS MINDS@UW
RD Aug 16, 2022