RT article
T1 MBW complexes impinge on anthocyanidin reductase gene regulation for proanthocyanidin biosynthesis in persimmon fruit
A1 Gil-Muñoz, Francisco
A1 Sánchez-Navarro, Jesús A.
A1 Besada, Cristina
A1 Salvador, Alejandra
A1 Badenes, María L.
A1 Naval, María M.
A1 Ríos, Gabino
K1 Agricultural genetics
K1 Plant development
K1 Secondary metabolism
K1 F30  Plant genetics and breeding
K1 Fruiting
K1 Plant breeding
AB MBW protein complexes containing MYB, bHLH and WD40 repeat factors are known transcriptional regulators of secondary metabolites production such as proanthocyanidins and anthocyanins, and developmental processes such as trichome formation in many plant species. DkMYB2 and DkMYB4 (MYB-type), DkMYC1 (bHLH-type) and DkWDR1 (WD40-type) factors have been proposed by different authors to take part of persimmon MBW complexes for proanthocyanidin accumulation in immature fruit, leading to its characteristic astringent flavour with important agronomical and ecological effects. We have confirmed the nuclear localization of these proteins and their mutual physical interaction by bimolecular fluorescence complementation analysis. In addition, transient expression of DkMYB2, DkMYB4 and DkMYC1 cooperatively increase the expression of a persimmon anthocyanidin reductase gene (ANR), involved in the biosynthesis of cis-flavan-3-ols, the structural units of proanthocyanidin compounds. Collectively, these data support the presence of MBW complexes in persimmon fruit and suggest their coordinated participation in ANR regulation for proanthocyanidin production.
PB Springer Nature
SN 2045-2322
YR 2020
FD 2020
LK http://hdl.handle.net/20.500.11939/6490
UL http://hdl.handle.net/20.500.11939/6490
LA en
NO Gil-Muñoz, F., Sánchez-Navarro, J. A., Besada, C., Salvador, A., Badenes, M. L., del Mar Naval, M., & Ríos, G. (2020). MBW complexes impinge on anthocyanidin reductase gene regulation for proanthocyanidin biosynthesis in persimmon fruit. Scientific reports, 10(1), 1-11.
DS MINDS@UW
RD Aug 16, 2022