RT article
T1 Unravelling the contribution of the Penicillium expansum PeSte12 transcription factor to virulence during apple fruit infection
A1 Sánchez-Torres, Paloma
A1 Vilanova, Laura
A1 Ballester, Ana Rosa
A1 López-Pérez, Mario
A1 Teixidó, Neus
A1 Vinas, Inmaculada
A1 Usall, Josep
A1 Gonzalez-Candelas, Luis
A1 Torres, Rosario
AB Blue mould disease caused by Penicillium expansum infection is one of the most important diseases of pome fruit accounting for important economic losses. In the present study, the PeSte12 transcription factor gene was identified, and deletant mutants were produced by gene replacement. Knockout mutants showed a significant decrease of virulence during apple fruit infection. Virulence was affected by the maturity stage of the fruit (immature, mature and over-mature), and disease severity was notably reduced when the apples were stored at 0 degrees C. The Delta PeSte12 mutants resulted defective in asexual reproduction, producing less conidia, but this characteristic did not correlate with differences in microscopic morphology. In addition, the DPeSte12 mutants produced higher quantity of hydrogen peroxide than the wild type strain. Gene expression analysis revealed that PeSte12 was induced over time during apple infection compared to axenic growth, particularly from 2 dpi, reinforcing its role in virulence. Analysis of transcriptional abundance of several genes in Delta PeSte12 mutants showed that in most of the evaluated genes, PeSte12 seemed to act as a negative regulator during axenic growth, as most of them exhibited an increasing expression pattern along the time period evaluated. The highest expression values corresponded to detoxification, ATPase activity, protein folding and basic metabolism. Gene expression analysis during apple infection showed that 3 out of 9 analysed genes were up regulated; thus, PeSte12 seemed to exert a positive control to particular type of aldolase. These results demonstrate the PeSte12 transcription factor could play an important role in P. expansum's virulence and asexual reproduction. (C) 2017 Elsevier Ltd. All rights reserved.
YR 2018
FD 2018
LK http://hdl.handle.net/20.500.11939/6241
UL http://hdl.handle.net/20.500.11939/6241
LA en
NO Sánchez-Torres, P.; Vilanova, L.; Rosa Ballester, A.; López-Pérez, M.; Teixido, N.; Vinas, I.; Usall, J.; Gonzalez-Candelas, L.; Torres, R. (2018). Unravelling the contribution of the penicillium expansum PeSte12 transcription factor to virulence during apple fruit infection. Food Microbiology, 69, 123-135.
DS MINDS@UW
RD Aug 18, 2022