RT article
T1 Recent Advances on Detection and Characterization of Fruit Tree Viruses Using High-Throughput Sequencing Technologies
A1 Maliogka, Varvara
A1 Minafra, Angelantonio
A1 Saldarelli, Pasquale
A1 Ruiz-García, Ana B.
A1 Glasa, Miroslav
A1 Katis, Nikolaos
A1 Olmos, Antonio
AB Perennial crops, such as fruit trees, are infected by many viruses, which are transmitted through vegetative propagation and grafting of infected plant material. Some of these pathogens cause severe crop losses and often reduce the productive life of the orchards. Detection and characterization of these agents in fruit trees is challenging, however, during the last years, the wide application of high-throughput sequencing (HTS) technologies has significantly facilitated this task. In this review, we present recent advances in the discovery, detection, and characterization of fruit tree viruses and virus-like agents accomplished by HTS approaches. A high number of new viruses have been described in the last 5 years, some of them exhibiting novel genomic features that have led to the proposal of the creation of new genera, and the revision of the current virus taxonomy status. Interestingly, several of the newly identified viruses belong to virus genera previously unknown to infect fruit tree species (e.g., Fabavirus, Luteovirus) a fact that challenges our perspective of plant viruses in general. Finally, applied methodologies, including the use of different molecules as templates, as well as advantages and disadvantages and future directions of HTS in fruit tree virology are discussed.
YR 2018
FD 2018
LK http://hdl.handle.net/20.500.11939/6223
UL http://hdl.handle.net/20.500.11939/6223
LA en
NO Maliogka, V.I.; Minafra, A.; Saldarelli, P.; Ruiz-Garcia, A. B.; Glasa, M.; Katis, N.; Olmos, A. (2018). Recent advances on detection and characterization of fruit tree viruses using high-throughput sequencing technologies. Viruses-Basel, 10(8), 436.
DS MINDS@UW
RD Aug 16, 2022