RT article
T1 Survey of huanglongbing associated with 'Candidatus Liberibacter' species in Spain: analyses of citrus plants and Trioza erytreae
A1 Siverio, Felipe
A1 Marco-Noales, Ester
A1 Bertolini, Edson
A1 Ribeiro Teresani, Gabriela
A1 Penyalver, Javier
A1 Mansilla, Pedro
A1 Aguin, Olga
A1 Perez-Otero, Rosa
A1 Abelleira, Adela
A1 Guerra-García, José
A1 Hernández-Suárez, Estrella
A1 Cambra, Mariano
A1 López, María M.
AB The disease huanglongbing (HLB), caused by the phloem-limited and psyllid-vectored 'Candidatus Liberibacter' spp., is threatening the Mediterranean citrus industry. The African psyllid (Trioza erytreae) vector of the pathogen was detected in Madeira (Portugal) in 1994 and in the Canary Islands (Spain) in 2002, and its arrival in 2014 in northwest Spain and Portugal along the Atlantic coast instigated a biological alert, and a contingency management plan was developed. Extensive surveys were conducted in Canary Islands from 2009 to 2015 and in the northwest mainland Spain (Galicia) since the first detection of T. erytreae. Symptoms of the psyllid were observed in most sweet orange orchards of five islands in Canary Islands (93% of the inspected plots). In northwest mainland Spain, 65% of the inspected plots up to 2016 showed T. erytreae symptoms. During the surveys, ten leaves / tree from trees showing suspicious symptoms and from symptomless trees, as well as adult psyllids, were collected and analysed by real-time PCR using a universal 'Ca. Liberibacter' spp. kit, according to the EPPO standard. Suspected samples from other surveyed Spanish regions free of the vector were also analysed. The few samples that were positive in the screening test were tested by species-specific real-time PCR protocols, and they did not show amplification. These data confirm that the Spanish citrus industry is currently free of the 'Ca. Liberibacter' spp., but strict measures to prevent the introduction of this pathogen are required as the presence of T. erytreae increases the risk of its dissemination.
SN 0031-9465
YR 2017
FD 2017
LK http://hdl.handle.net/20.500.11939/6101
UL http://hdl.handle.net/20.500.11939/6101
LA en
NO Siverio, F., Marco-Noales, E., Bertolini, E., Ribeiro Teresani, G., Penalver, J., Mansilla, P. et al. (2017). Survey of huanglongbing associated with 'candidatus liberibacter' species in spain: Analyses of citrus plants and trioza erytreae. Phytopathologia Mediterranea, 56(1), 98-110.
DS MINDS@UW
RD Feb 8, 2023