RT article
T1 Dual regulation of water retention and cell growth by a stressassociated protein (SAP) gene in Prunus
A1 Lloret, Alba
A1 Conejero, Ana
A1 Leida, Carmen
A1 Petri, Cesar
A1 Gil-Muñoz, Francisco
A1 Burgos, Lorenzo
A1 Badenes, María L.
A1 Ríos, Gabino
AB We have identified a gene (PpSAP1) of Prunus persica coding for a stress-associated protein (SAP) containing Zn-finger domains A20 and AN1. SAPs have been described as regulators of the abiotic stress response in plant species, emerging as potential candidates for improvement of stress tolerance in plants. PpSAP1 was highly expressed in leaves and dormant buds, being down-regulated before bud dormancy release. PpSAP1 expression was moderately induced by water stresses and heat in buds. In addition, it was found that PpSAP1 strongly interacts with polyubiquitin proteins in the yeast two-hybrid system. The overexpression of PpSAP1 in transgenic plum plants led to alterations in leaf shape and an increase of water retention under drought stress. Moreover, we established that leaf morphological alterations were concomitant with a reduced cell size and down-regulation of genes involved in cell growth, such as GROWTH-REGULATING FACTOR (GRF) 1-like, TONOPLAST INTRINSIC PROTEIN (TIP)-like, and TARGET OF RAPAMYCIN (TOR)-like. Especially, the inverse expression pattern of PpSAP1 and TOR-like in transgenic plum and peach buds suggests a role of PpSAP1 in cell expansion through the regulation of TOR pathway.
SN 2045-2322
YR 2017
FD 2017
LK http://hdl.handle.net/20.500.11939/6060
UL http://hdl.handle.net/20.500.11939/6060
LA en
NO Lloret, A., Conejero, A., Leida, C., Petri, C., Gil-Munoz, F., Burgos, L., Badenes, M. L., Rios, G. (2017). Dual regulation of water retention and cell growth by a stressassociated protein (SAP) gene in prunus. Scientific Reports, 7, 332.
DS MINDS@UW
RD Aug 7, 2022