RT article T1 Ejaculation does not contribute to the stress response toelectroejaculation in sheep A1 Abril-Sanchez, S. A1 Freitas-de-Melo, A. A1 Damian, J.P. A1 Giriboni, J. A1 Villagra, Arantxa A1 Ungerfeld, R. AB Contents Electroejaculation procedures (EEPs) provoke stress; nevertheless, ejaculation produces physiological changes similar as those usually used to measure stress responses. The application of EEP to animals that cannot ejaculateas ewesmay be useful to discriminate the responses induced by ejaculation from those provoked by EEP. The aim was to determine the stress response to EEP in rams and ewes. The EEPs were applied to 10 rams and 10 ewes during the non-breeding season, and the number of vocalizations, the heart rate, rectal temperature, serum cortisol concentration, biochemical and haematological parameters were measured. Overall, EEP provoked increases in cortisol concentration, glycaemia, rectal temperature and concentration of creatine kinase (all them: p<.0001) as well as relative concentration of granulocytes (p=.003) and absolute granulocyte concentration (p=.0002) in both, rams and ewes. Heart rate, relative concentration of lymphocytes (p=.001), haematocrit (p=.02) and haemoglobin (p=.045) decreased in animals from both genders after EEP. Besides, cortisol (p<.0001), rectal temperature (p=.002) and glycaemia (p=.001) were greater in ewes than rams, and creatine kinase also tended to be greater in ewes than rams (p=.054). On the other hand, the number of animals that vocalized (p=.006), white blood cells (p=.02) and absolute lymphocytes (p=.02) were greater in rams than ewes. The general trends show a similar pattern of stress responses in animals from both genders. Therefore, we concluded that ejaculation does not contribute to the stress response provoked by the EEP. This procedure also provokes muscular damage and probably pain. SN 0936-6768 YR 2017 FD 2017 LK http://hdl.handle.net/20.500.11939/6014 UL http://hdl.handle.net/20.500.11939/6014 LA en NO Abril-Sanchez, S., Freitas-de-Melo, A., Damian, J. P., Giriboni, J., Villagra-Garcia, A., Ungerfeld, R. (2017). Ejaculation does not contribute to the stress response toelectroejaculation in sheep. Reproduction in Domestic Animals, 52(3), 403-408. DS MINDS@UW RD Jun 27, 2022