RT article
T1 Detection and absolute quantitation of Broad bean wilt virus 1 (BBWV-1) and BBWV-2 by real time RT-PCR
A1 Ferriol, Inmaculada
A1 Ruiz-Ruiz, Susana
A1 Rubio, Luis
AB Broad bean wilt virus 1 (BBWV-1) and BBWV-2 are the two most significant viruses in the genus Fabavirus, causing damage to many economically important agricultural crops worldwide. A quantitative real-time reverse transcription-polymerase chain reaction (RT-qPCR) procedure using two TaqMan (R) MGB probes was developed for sensitive and specific detection and quantitation of BBWV-1 and BBWV-2. Primers and probes were designed from conserved sequence stretches to detect all isolates of each virus. Standard curves using RNA transcripts identical to both TaqMan (R) MGB probes enabled absolute quantitation, with a wide dynamic range and high sensitivity (10(3)-10(10) RNA molecules). RT-qPCR was assayed with genetically divergent BBWV-1 and BBWV-2 isolates from different plant hosts and countries, and was used to evaluate the temporal accumulation of BBWV-1 RNA in two plant hosts. (C) 2011 Elsevier B.V. All rights reserved.
SN 0166-0934
YR 2011
FD 2011
LK http://hdl.handle.net/20.500.11939/5203
UL http://hdl.handle.net/20.500.11939/5203
UL http://hdl.handle.net/20.500.11939/5203
LA en
NO Ferriol, I., Ruiz-Ruiz, S., Rubio, L. (2011). Detection and absolute quantitation of Broad bean wilt virus 1 (BBWV-1), and BBWV-2 by real time RT-PCR. Journal of virological methods, 177(2), 202-205.
DS MINDS@UW
RD Dec 2, 2023