RT article
T1 Comparative study of the protein profiles of Sunki mandarin and Rangpur lime plants in response to water deficit
A1 Oliveira, Tahise M.
A1 da Silva, Fernanda R.
A1 Bonatto, Diego
A1 Neves, Diana M.
A1 Morillon, Raphael
A1 Maserti, Bianca E.
A1 Coelho Filho, Mauricio A.
A1 Costa, Marcio G. C.
A1 Pirovani, Carlos P.
A1 Gesteira, Abelmon S.
K1 Citrus rootstock
K1 Water deficit
K1 Proteomics
K1 Protein network
K1 Drought stress
K1 Abscisic-acid
K1 Interaction networks
K1 Oxidative stress
K1 Proteomic analysis
K1 Mass-spectrometry
K1 Abiotic stress
K1 Gene
K1 Arabidopsis
K1 Tolerance
AB Background: Rootstocks play a major role in the tolerance of citrus plants to water deficit by controlling andadjusting the water supply to meet the transpiration demand of the shoots. Alterations in protein abundance incitrus roots are crucial for plant adaptation to water deficit. We performed two-dimensional electrophoresis (2-DE)separation followed by LC/MS/MS to assess the proteome responses of the roots of two citrus rootstocks, Rangpurlime (Citrus limonia Osbeck) and ‘Sunki Maravilha’ (Citrus sunki) mandarin, which show contrasting tolerances towater deficits at the physiological and molecular levels.Results: Changes in the abundance of 36 and 38 proteins in Rangpur lime and ‘Sunki Maravilha’ mandarin,respectively, were observed via LC/MS/MS in response to water deficit. Multivariate principal componentanalysis (PCA) of the data revealed major changes in the protein profile of ‘Sunki Maravilha’ in response towater deficit. Additionally, proteomics and systems biology analyses allowed for the general elucidation of themajor mechanisms associated with the differential responses to water deficit of both varieties. The defensemechanisms of Rangpur lime included changes in the metabolism of carbohydrates and amino acids as well asin the activation of reactive oxygen species (ROS) detoxification and in the levels of proteins involved in waterstress defense. In contrast, the adaptation of ‘Sunki Maravilha’ to stress was aided by the activation of DNA repairand processing proteins.Conclusions: Our study reveals that the levels of a number of proteins involved in various cellular pathways areaffected during water deficit in the roots of citrus plants. The results show that acclimatization to water deficitinvolves specific responses in Rangpur lime and ‘Sunki Maravilha’ mandarin. This study provides insights into theeffects of drought on the abundance of proteins in the roots of two varieties of citrus rootstocks. In addition,this work allows for a better understanding of the molecular basis of the response to water deficit in citrus.Further analysis is needed to elucidate the behaviors of the key target proteins involved in this response.
SN 1471-2229
YR 2015
FD 2015
LK http://hdl.handle.net/20.500.11939/4236
UL http://hdl.handle.net/20.500.11939/4236
UL http://hdl.handle.net/20.500.11939/4236
LA en
NO Oliveira, T. M., da Silva, F. R., Bonatto, D., Neves, D. M., Morillon, R., Maserti, B. E. et al.(2015). Comparative study of the protein profiles of Sunki mandarin and Rangpur lime plants in response to water deficit. Bmc Plant Biology, 15, 69-69.
DS MINDS@UW
RD Aug 9, 2022