RT article
T1 Detection and absolute quantitation of Tomato torrado virus (ToTV) by real time RT-PCR
A1 Herrera-Vásquez, José A.
A1 Rubio, Luis
A1 Alfaro-Fernández, Ana
A1 Debreczeni, Diana E.
A1 Font-San-Ambrosio, Isabel
A1 Falk, Bryce W.
A1 Ferriol, Inmaculada
AB Tomato torrado virus (ToTV) causes serious damage to the tomato industry and significant economic losses. A quantitative real-time reverse-transcription polymerase chain reaction (RT-qPCR) method using primers and a specific TaqMan (R) MGB probe for ToTV was developed for sensitive detection and quantitation of different ToTV isolates. A standard curve using RNA transcripts enabled absolute quantitation, with a dynamic range from 10(4) to 10(10) ToTV RNA copies/ng of total RNA. The specificity of the RT-qPCR was tested with twenty-three ToTV isolates from tomato (Solanum lycopersicum L), and black nightshade (Solanum nigrum L) collected in Spain, Australia, Hungary and France, which covered the genetic variation range of this virus. This new RT-qPCR assay enables a reproducible, sensitive and specific detection and quantitation of ToTV, which can be a valuable tool in disease management programs and epidemiological studies.
SN 0166-0934
YR 2015
FD 2015
LK http://hdl.handle.net/20.500.11939/4206
UL http://hdl.handle.net/20.500.11939/4206
LA en
NO Angel Herrera-Vasquez, J., Rubio, L., Alfaro-Fernandez, Ana, Elvira Debreczeni, Diana, Font-San-Ambrosio, I., Falk, Bryce W., Ferriol, I. (2015). Detection and absolute quantitation of Tomato torrado virus (ToTV), by real time RT-PCR. Journal of virological methods, 221, 90-94.
DS MINDS@UW
RD Aug 15, 2022