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dc.contributor.authorViudes-De-Castro, María P. 
dc.contributor.authorMarco-Jiménez, Francisco
dc.contributor.authorSalvador Vicente, José
dc.date.accessioned2023-07-19T09:55:28Z
dc.date.available2023-07-19T09:55:28Z
dc.date.issued2023es
dc.identifier.citationViudes-de-Castro, M. P., Marco-Jiménez, F. & Salvador-Vicente, J. S. (2023). Reproductive Performance of Female Rabbits Inseminated with Extenders Supplemented with GnRH Analogue Entrapped in Chitosan-Based Nanoparticles. Animals, 13(10), 1628.es
dc.identifier.issn2076-2615
dc.identifier.urihttps://hdl.handle.net/20.500.11939/8679
dc.description.abstractRabbit is a reflexively ovulating species. Accordingly, in the practice of artificial insemination (AI) ovulation must be induced via exogenous GnRH (Gonadotropin-Releasing Hormone) administration, which may be performed intramuscularly, subcutaneously, or intravaginally. Unfortunately, the bioavailability of the GnRH analogue when added to the extender is lower due to the proteolytic activity in the seminal plasma and the poor permeability of the vaginal mucosa. The aim of the study was to refine the practice of AI practice in rabbits by replacing parenteral GnRH analogue administration (subcutaneous, intravenous, or intramuscular injection) with intravaginal application, while reducing its concentration in the diluent. Extenders containing the buserelin acetate in chitosan–dextran sulphate and chitosan–alginate nanoparticles were designed and 356 females were inseminated. Reproductive performance of females inseminated with the two experimental extenders, receiving 4 μg of buserelin acetate intravaginally per doe, was compared with that in the control group, the does of which were inseminated with the extender without the GnRH analogue and induced to ovulate with 1 μg of buserelin acetate administered intramuscularly. The entrapment efficiency of the chitosan–dextran sulphate complex was higher than that of chitosan–alginate. However, females inseminated with both systems showed similar reproductive performance. We conclude that both nanoencapsulation systems are an efficient way of intravaginal ovulation induction, allowing a reduction in the level of the GnRH analogue normally used in seminal doses from 15–25 μg to 4 μg.es
dc.language.isoenes
dc.publisherMDPIes
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectNanoencapsulationes
dc.subjectDextran sulphatees
dc.titleReproductive Performance of Female Rabbits Inseminated with Extenders Supplemented with GnRH Analogue Entrapped in Chitosan-Based Nanoparticleses
dc.typearticlees
dc.authorAddressviudes_mar@gva.eses
dc.entidadIVIACentro de Tecnología Animales
dc.identifier.doi10.3390/ani13101628es
dc.identifier.urlhttps://www.mdpi.com/2076-2615/13/10/1628es
dc.journal.issueNumber10es
dc.journal.titleAnimalses
dc.journal.volumeNumber13es
dc.page.final1628es
dc.page.initial1628es
dc.relation.projectIDThis research was funded through grant PID2021-127867OB-100 by MCIN/AEI/10.13039/ 501100011033 and by ERDF, “A way of making Europe”, and through grant 52201K by GVA-IVIA and the FEDER Operational Program of the Valencian Community, 2021–2027.es
dc.relation.projectIDinfo:eu-repo/grantAgreement/ERDF/PCV 2021-2027/52201/ES/Relanzando la agroalimentación valenciana para una producción y consumo sostenibles y seguros/AgroAlimVales
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Programa Estatal de Generación de Conocimiento y Fortalecimiento Científico y Tecnológico del Sistema de I+D+i/PID2021-127867OB-100es
dc.rights.accessRightsopenAccesses
dc.source.typeelectronicoes
dc.subject.agrovocRabbits es
dc.subject.agrovocArtificial insemination es
dc.subject.agrovocChitosan es
dc.subject.agrovocAlginates es
dc.subject.agrovocInduced ovulation es
dc.subject.agrovocReproductive performance es
dc.type.hasVersionpublishedVersiones


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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