Effect of Sperm Concentration and Storage Temperature on Goat Spermatozoa during Liquid Storage

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URI
https://hdl.handle.net/20.500.11939/8583
DOI
10.3390/biology9090300
URL
https://www.mdpi.com/2079-7737/9/9/300
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openAccess
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Author
Sadeghi, Sara; Del-Gallego, Raquel; García-Colomer, Balma; Gómez, Ernesto A.Autoridad IVIA; Yániz, Jesús L.; Gosálvez, Jaime; López-Fernández, Carmen; Silvestre, Miguel A.
Date
2020
Cita bibliográfica
Sadeghi, S., Del-Gallego, R., García-Colomer, B., Gómez, E. A., Yániz, J. L., Gosálvez, J.et al. (2020). Effect of sperm concentration and storage temperature on goat spermatozoa during liquid storage. Biology, 9(9), 300.
Abstract
One of the problems with the refrigerated storage of spermatozoa in goats is the short shelf life. The study of the effect of the different storage conditions on the di erent sperm parameters could help to increase sperm survival. In this work, we studied the effect of temperature and sperm concentration during refrigerated storage on sperm motility, mitochondrial membrane potential and DNA fragmentation. We observed that refrigerated storage of semen doses reduced the majority of sperm quality parameters, however, sperm DNA fragmentation was not a ected. Storage at 5ºC preserved higher sperm motility than at 17ºC. Moreover, the reduction of sperm concentration below 500x10(6) sperm/mL did not seem to improve the quality of spermatozoa.
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