HKT1;1 and HKT1;2 Na+ Transporters from Solanum galapagense Play Different Roles in the Plant Na+ Distribution under Salinity
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AuthorAsins, María J.; Romero-Aranda, María R.; Espinosa, Jesús; González-Fernández, Paloma; Jaime-Fernández, Emilio; Traverso, Jose A.; Carbonell, Emilio A.; Belver, Andres
Cita bibliográficaAsins, M. J., Romero-Aranda, M. R., Espinosa, J., González-Fernández, P., Jaime-Fernández, E., Traverso, J. A. et al. (2022). HKT1; 1 and HKT1; 2 Na+ Transporters from Solanum galapagense Play Different Roles in the Plant Na+ Distribution under Salinity. International Journal of Molecular Sciences, 23(9), 5130.
Salt tolerance is a target trait in plant science and tomato breeding programs. Wild tomato accessions have been often explored for this purpose. Since shoot Na+/K+ is a key component of salt tolerance, RNAi-mediated knockdown isogenic lines obtained for Solanum galapagense alleles encoding both class I Na+ transporters HKT1;1 and HKT1;2 were used to investigate the silencing effects on the Na and K contents of the xylem sap, and source and sink organs of the scion, and their contribution to salt tolerance in all 16 rootstock/scion combinations of non-silenced and silenced lines, under two salinity treatments. The results show that SgHKT1;1 is operating differently from SgHKT1;2 regarding Na circulation in the tomato vascular system under salinity. A model was built to show that using silenced SgHKT1;1 line as rootstock would improve salt tolerance and fruit quality of varieties carrying the wild type SgHKT1;2 allele. Moreover, this increasing effect on both yield and fruit soluble solids content of silencing SgHKT1;1 could explain that a low expressing HKT1;1 variant was fixed in S. lycopersicum during domestication, and the paradox of increasing agronomic salt tolerance through silencing the HKT1;1 allele from S. galapagense, a salt adapted species.