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dc.contributor.authorLenarcic, Rok
dc.contributor.authorMorisset, Dany
dc.contributor.authorPirc, Mnaca
dc.contributor.authorLlop, Pablo
dc.contributor.authorRavnikar, Maja
dc.contributor.authorDreo, Tanja
dc.date.accessioned2022-04-29T16:21:20Z
dc.date.available2022-04-29T16:21:20Z
dc.date.issued2014es
dc.identifier.citationLenarčič, R., Morisset, D., Pirc, M., Llop, P., Ravnikar, M. & Dreo, T. (2014). Loop-mediated isothermal amplification of specific endoglucanase gene sequence for detection of the bacterial wilt pathogen Ralstonia solanacearum. PLoS One, 9(4), e96027.es
dc.identifier.issn1932-6203 (eISSN)
dc.identifier.urihttp://hdl.handle.net/20.500.11939/8062
dc.description.abstractThe increased globalization of crops production and processing industries also promotes the side-effects of more rapid and efficient spread of plant pathogens. To prevent the associated economic losses, and particularly those related to bacterial diseases where their management relies on removal of the infected material from production, simple, easy-to-perform, rapid and cost-effective tests are needed. Loop-mediated isothermal amplification (LAMP) assays that target 16S rRNA, fliC and egl genes were compared and evaluated as on-site applications. The assay with the best performance was that targeted to the egl gene, which shows high analytical specificity for diverse strains of the betaproteobacterium Ralstonia solanacearum, including its non-European and non-race 3 biovar 2 strains. The additional melting curve analysis provides confirmation of the test results. According to our extensive assessment, the egl LAMP assay requires minimum sample preparation (a few minutes of boiling) for the identification of pure cultures and ooze from symptomatic material, and it can also be used in a high-throughput format in the laboratory. This provides sensitive and reliable detection of R. solanacearum strains of different phylotypes.es
dc.language.isoenes
dc.publisherPLOSes
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectLoop-mediated isothermal amplificationes
dc.titleLoop-Mediated Isothermal Amplification of Specific Endoglucanase Gene Sequence for Detection of the Bacterial Wilt Pathogen Ralstonia solanacearumes
dc.typearticlees
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.entidadIVIACentro de Protección Vegetal y Biotecnologíaes
dc.identifier.doi10.1371/journal.pone.0096027es
dc.identifier.urlhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0096027es
dc.journal.issueNumber4es
dc.journal.titlePLoS Onees
dc.journal.volumeNumber9es
dc.page.finale96027es
dc.page.initiale96027es
dc.relation.projectIDThis study was carried out under the sponsorship of the EU Framework 7 Programme (FP7-KBBE-2009-3) project 245047 (Qdetect – Developing quarantine pest detection methods for use by national plant protection organisations – NPPO and inspection services) and of the Slovenian Research Agency funded project Q-finder L1-3642 (Developing simple, rapid and on-site methods for plant pathogens detection).es
dc.rights.accessRightsopenAccesses
dc.source.typeelectronicoes
dc.subject.agrisU30 Research methodses
dc.subject.agrisH20 Plant diseaseses
dc.subject.agrovocGene sequencees
dc.subject.agrovocRalstonia solanacearumes
dc.subject.agrovocDiagnostic techniqueses
dc.subject.agrovocPCRes
dc.subject.agrovocTestinges
dc.type.hasVersionpublishedVersiones


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