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dc.contributor.authorGolmohammadi, Morteza
dc.contributor.authorCubero, Jaime
dc.contributor.authorPenyalver, Jaime
dc.contributor.authorQuesada, Jose M.
dc.contributor.authorLópez, María M. 
dc.contributor.authorLlop, Pablo 
dc.date.accessioned2022-04-29T16:20:44Z
dc.date.available2022-04-29T16:20:44Z
dc.date.issued2007es
dc.identifier.citationGolmohammadi, M., Cubero, J., Peñalver, J., Quesada, J. M., López, M. M. & Llop, P. (2007). Diagnosis of Xanthomonas axonopodis pv. citri, causal agent of citrus canker, in commercial fruits by isolation and PCR‐based methods. Journal of Applied Microbiology, 103(6), 2309-2315.es
dc.identifier.issn1364-5072
dc.identifier.urihttp://hdl.handle.net/20.500.11939/8060
dc.description.abstractAims: To show the results of the detection of an EU quarantine organism, Xanthomonas axonopodis pv. citri (Xac), in citrus fruits imported from countries where this bacterium is present, using an integrated approach that includes isolation, pathogenicity assays and molecular techniques. Methods and Results: Citrus fruits with canker-like symptoms, exported to Spain from South American countries were analysed by several methods. Bacterial isolation, three conventional polymerase chain reaction (PCR) protocols, and real-time PCR with SYBR Green or a TaqMan probe, were compared. Canker-like lesions were disrupted in PBS buffer, and the extract used for bacterial isolation and DNA extraction followed by PCR amplification. Canker lesions, identified by PCR, showed viable bacteria in eleven of fifteen fruit samples. In 16 out of 130 lesions analysed from these samples, Xac was isolated, and pathogenicity on grapefruit leaves confirmed. By real-time PCR, using SYBR green or a Taqman probe, Xac was detected in 58 and 80 lesions respectively. By conventional PCR the bacterium was detected in 39–52 lesions depending on the protocol employed. Conclusions: An integrated approach for reliable detection of Xac in lesions of fruit samples, employing several techniques and with real-time PCR using a TaqMan probe as the fastest and most sensitive screening method, has been established and validated and is proposed as a useful tool for the analysis of Xac on fresh fruits. Significance and Impact of the Study: This work faces up to the real threat of the importation of citrus fruits that can harbour quarantine bacteria and will be useful in diagnostic laboratories for the analysis of commercial fresh fruits from countries where citrus canker is present.es
dc.language.isoenes
dc.publisherSociety for Applied Microbiologyes
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectValidationses
dc.subjectReal time-PCRes
dc.subjectSensitivityes
dc.subjectXanthomonas axonopodises
dc.titleDiagnosis of Xanthomonas axonopodis pv. citri, causal agent of citrus canker, in commercial fruits by isolation and PCR-based methodses
dc.typearticlees
dc.authorAddressllop_pab@gva.eses
dc.entidadIVIACentro de Protección Vegetal y Biotecnologíaes
dc.identifier.doi10.1111/j.1365-2672.2007.03484.xes
dc.identifier.urlhttps://sfamjournals.onlinelibrary.wiley.com/doi/epdf/10.1111/j.1365-2672.2007.03484.xes
dc.journal.issueNumber6es
dc.journal.titleJournal of Applied Microbiologyes
dc.journal.volumeNumber03es
dc.page.final2315es
dc.page.initial2309es
dc.relation.projectIDThis research was supported by the agreement between the Ministerio de Agricultura of Spain, Subdirección General de Sanidad Vegetal and IVIA, through the project INIA RTA2006-00149-00-00, and by Plan de Vigilancia Fitosanitaria of the Generalitat Valenciana.es
dc.rights.accessRightsopenAccesses
dc.source.typeelectronicoes
dc.subject.agrisH20 Plant diseaseses
dc.subject.agrisJ10 Handling, transport, storage and protection of agricultural productses
dc.subject.agrisE71 International tradees
dc.subject.agrovocprotocols es
dc.subject.agrovocsensitivityes
dc.subject.agrovocCitrus fruits es
dc.subject.agrovocDiagnosis es
dc.subject.agrovocexportes
dc.type.hasVersionpublishedVersiones


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