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dc.contributor.authorCubero, Jaime
dc.contributor.authorMartínez, M. Carmen
dc.contributor.authorLlop, Pablo
dc.contributor.authorLópez, María M.
dc.date.accessioned2022-04-29T16:12:58Z
dc.date.available2022-04-29T16:12:58Z
dc.date.issued2001es
dc.identifier.citationCubero, J., Martinez, M. C., Llop, P. & López, M. M. (1999). A simple and efficient PCR method for the detection of Agrobacterium tumefaciens in plant tumours. Journal of Applied Microbiology, 86(4), 591-602.es
dc.identifier.issn1365-2389 (eISSN)
dc.identifier.urihttp://hdl.handle.net/20.500.11939/8056
dc.description.abstractA simple PCR protocol was developed for identifying Agrobacterium as the causal agent of the tumours produced by this bacterium in plant material. The sensitivity of this method was compared with that of bacterial isolation using common and selective media with a previous enrichment step. More than 200 samples from tumours of naturally infected and inoculated plants from several hosts including almond, peach×almond hybrids, apricot, rose, tobacco, tomato, raspberry, grapevine and chrysanthemum, were analysed by both methods. PCR was the most efficient method for detecting the bacterial aetiology of the plant tumours. Agrobacterium tumefaciens was better detected in crown and root tumours than in aerial tumours with all the methods assayed in inoculated plants. A comparison between the efficiency of the diagnosis by analysing pieces from the external and internal part of the tumour showed no differences between them.es
dc.language.isoenes
dc.publisherSociety for Applied Microbiologyes
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.titleA simple and efficient PCR method for the detection of Agrobacterium tumefaciens in plant tumourses
dc.typearticlees
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.entidadIVIACentro de Protección Vegetal y Biotecnologíaes
dc.identifier.doi10.1046/j.1365-2672.1999.00700.xes
dc.identifier.urlhttps://sfamjournals.onlinelibrary.wiley.com/doi/epdf/10.1046/j.1365-2672.1999.00700.xes
dc.journal.issueNumber4es
dc.journal.titleJournal of Applied Microbiologyes
dc.journal.volumeNumber86es
dc.page.final602es
dc.page.initial591es
dc.relation.projectIDThis research was grant funded by projects 93–117 of the INIA and by contract IC18-CT97–0198 of the INCO-DC programme of the European Community.es
dc.rights.accessRightsopenAccesses
dc.source.typeelectronicoes
dc.subject.agrisH20 Plant diseaseses
dc.subject.agrisU30 Research methodses
dc.subject.agrovocPCRes
dc.subject.agrovocAgrobacteriumes
dc.subject.agrovocDiagnostic techniqueses
dc.subject.agrovocPlant tumourses
dc.type.hasVersionpublishedVersiones


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