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dc.contributor.authorGolmohammadi, Morteza
dc.contributor.authorCubero, Jaime
dc.contributor.authorLópez, María M.
dc.contributor.authorLlop, Pablo
dc.date.accessioned2022-03-30T10:31:01Z
dc.date.available2022-03-30T10:31:01Z
dc.date.issued2013es
dc.identifier.citationGolmohammadi, M., Cubero, J., Lopez, M. M. & Llop, P. (2013). The viable but non-culturable state in Xanthomonas citri subsp. citri is a reversible state induced by low nutrient availability and copper stress conditions. Journal of Life Sciences, 7(10), 1019-1029.es
dc.identifier.issn1934-7391
dc.identifier.issn1934-7405 (on-line)
dc.identifier.urihttp://hdl.handle.net/20.500.11939/8005
dc.description.abstractXcc (Xanthomonas citri subsp. citri) causes citrus bacterial canker, a leaf, stem and fruit spotting disease that affects most commercial citrus species and cultivars. Copper compounds, widely used for management of this pathogen, have been reported as inducers of a VBNC (viable but non-culturable state) in plant pathogenic bacteria. VBNC may be considered as a state preceding bacterial death or as a survival mechanism under adverse conditions. Several experiments were performed to characterize the reversibility and persistence of the VBNC state in Xcc. VBNC was induced in low nutrient medium or with amendment of copper at concentrations used for field disease control. The VBNC condition was demonstrated to persist up to 150 days after copper treatment and was reversed after the addition of culture media without copper or amendment with citrus leaf extract. Xcc viability was evaluated by recovery of colonies on culture media, confirmed by membrane integrity, respiratory activity and by real-time RT-PCR targeting a sequence from the gumD gene. Besides, the colonies recovered were pathogenic on citrus leaves. These results confirm that the VBNC state in Xcc is inducible and reversible and therefore may occur in the phyllosphere when Xcc is under copper stress or starvation.es
dc.language.isoenes
dc.publisherDavid Publishinges
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectVBNC (viable but non-culturable state)es
dc.subjectCopper stresses
dc.subjectLow nutrient availabilityes
dc.subjectCitrus bacterial cankeres
dc.titleThe Viable but Non-culturable State in Xanthomonas citri subsp. citri is a Reversible State Induced by Low Nutrient Availability and Copper Stress Conditionsen
dc.typearticlees
dc.authorAddressllop_pab@gva.eses
dc.entidadIVIACentro de Protección Vegetal y Biotecnologíaes
dc.identifier.urlhttps://www.davidpublisher.com/index.php/Home/Article/index?id=9795.htmles
dc.journal.issueNumber10es
dc.journal.titleJournal of Life Scienceses
dc.journal.volumeNumber7es
dc.page.final1029es
dc.page.initial1019es
dc.relation.projectIDThis research was supported by INIA projects RTA2006-00149 and RTA2008-00048.es
dc.rights.accessRightsopenAccesses
dc.source.typeelectronicoes
dc.subject.agrisH20 Plant diseaseses
dc.subject.agrisU30 Research methodses
dc.subject.agrovocResuscitationes
dc.subject.agrovocXanthomonas citries
dc.subject.agrovocCitruses
dc.type.hasVersionpublishedVersiones


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