Incidence of Citrus tristeza virus in Chile and Biological and Serological Characterization of 100 Isolates
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AuthorBesoain, X. A.; Simpson, M. C.; Ramella, F.; Canales, Celia; Valenzuela, M.; Castro, M.; Gorris, María T.; Cambra, Mariano
Cita bibliográficaBesoain, X. A., Simpson, M. C., Ramella, F., Canales, C., Valenzuela, M., Castro, M. et al. (2005). Incidence of Citrus tristeza virus in Chile and Biological and Serological Characterization of 100 Isolates. Proceedings of the Sixteenth Conference of the International Organization of Citrus Virologists, 416-418.
A survey was performed from 1999 to 2002 in the main citrus regions (13,551 ha) of Chile to estimate the Citrus tristeza virus(CTV) incidence. One thousand three hundred and twenty-three commercial citrus orchards were visited and 90,267 trees were observed for symptoms, sampled and analyzed by DAS-ELISA and tissue print-ELISA using 3DF1 and 3CA5 monoclonal antibodies. In this survey stem pitting symptoms were detected on CTV-infected grapefruit and Mexican lime trees grown in small orchards located in the Pica Oasis where the CTV incidence was 17.2%. In central Chile, where the main commercial orchards are located, CTV incidence ranged from zero to 0.37%. The average incidence of CTV in Chile was estimated at 0.38%. From this survey 100 CTV isolates representative of different hosts and geographical origins were selected and inoculated on sweet orange and/or Mexican lime and conserved in a collection kept in screenhouse facilities. The isolates in the collection were biologically and serologically characterized. Severe CTV isolates were found in Region I (where Pica Oasis is located). Serological characterization was done by DASI-ELISA using 12 different monoclonal antibodies, including MCA 13. Eight different serogroups were detected, two of which included CTV isolates which yielded a positive MCA 13 reaction.