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dc.contributor.authorBernad, Lucia
dc.contributor.authorDurán-Vila, Núria
dc.contributor.editorHilf, M. E.
dc.date.accessioned2021-10-27T14:41:59Z
dc.date.available2021-10-27T14:41:59Z
dc.date.issued2005es
dc.identifier.citationBernad, L. & Durán-Vila, N. (2005). An Improved Protocol for Extraction and RT-PCR Detection of Citrus Viroids. Proceedings of the Sixteenth Conference of the International Organization of Citrus Virologists, 452-455.es
dc.identifier.issn2313-5123
dc.identifier.issn0074-7203 (e-ISSN)
dc.identifier.urihttp://hdl.handle.net/20.500.11939/7703
dc.description.abstractThe great sensitivity of RT-PCR makes this technique a desirable alternative to other diagnostic methods for detection of viroids. In previous studies, difficulties have been encountered in obtaining amplicons from samples known to contain viroids. The aim of the present study was to minimize extraction procedures and to design RT-PCR protocols based on the use of thermostable reverse transcriptases. The use of 27-nucleotide primers and reverse transcription at 60°C minimized renaturation of the viroid template and consistently yielded the expected PCR products for each viroid. The protocol has been tested with samples from a large number of viroid-host combinations grown under greenhouse conditions as well as with samples from the field.es
dc.language.isoenes
dc.publisherIOCVes
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectRT-PCRes
dc.subjectDiagnosis methodses
dc.subjectViroidses
dc.subjectThermostable reverse transcriptaseses
dc.subjectCitrus viroidses
dc.titleAn Improved Protocol for Extraction and RT-PCR Detection of Citrus Viroidsen
dc.typeconferenceObjectes
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.entidadIVIACentro de Protección Vegetal y Biotecnologíaes
dc.identifier.urlhttps://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/452-455%20IOCV%2016.pdfes
dc.page.final455es
dc.page.initial452es
dc.relation.conferenceDate2004-11-07
dc.relation.conferenceNameSixteenth Conference of the International Organization of Citrus Virologistses
dc.relation.conferencePlaceMonterrey, Mexicoes
dc.rights.accessRightsopenAccesses
dc.source.typeelectronicoes
dc.subject.agrisH20 Plant diseaseses
dc.subject.agrisU30 Research methodses


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Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 España