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dc.contributor.authorVilanova, Laura
dc.contributor.authorTeixidó, Neus
dc.contributor.authorTorres, Rosario
dc.contributor.authorUsall, Josep
dc.contributor.authorVinas, Inmaculada
dc.contributor.authorSánchez-Torres, Paloma
dc.identifier.citationVilanova, L., Teixidó, N., Torres, R., Usall, J., Viñas, I., & Sánchez-Torres, P. (2016). Relevance of the transcription factor PdSte12 in Penicillium digitatum conidiation and virulence during citrus fruit infection. International journal of food microbiology, 235,
dc.description.abstractGreen mould, resulting from Penicillium digitatum, is the most important postharvest disease of citrus. In a previous study, the PdSte12 transcription factor gene was identified, and disruption mutants were obtained. In the present study, the ΔPdSte12 mutants generated through gene replacement showed significantly reduced virulence during citrus fruit infection. Virulence was affected not only in mature fruit but also in immature fruit, and disease severity was markedly reduced when the oranges were stored at 20 or 4 °C. In addition, the ΔPdSte12 mutants were defective in asexual reproduction, producing few conidia. The conidiophores of these mutants had longer metulae with fewer branches at the tip of the hyphae. Gene expression analysis revealed that PdSte12 might act as a negative regulator of several transporter-encoding genes and a positive regulator of two sterol demethylases, all of which are involved in fungicide resistance and fungal virulence. Moreover, PdSte12 exhibited the negative regulation of another transcription factor PdMut3, putatively involved in fungal pathogenesis but with no effect on the MAPK SLT2 P. digitatum orthologue belonging to different transcription pathways relevant to cell integrity. These results indicate the PdSte12 transcription factor is functionally conserved in P. digitatum for infection and asexual reproduction, similar to other Ste12 fungal plant
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.subjectFruit-pathogen interactiones
dc.titleRelevance of the transcription factor PdSte12 in Penicillium digitatum conidiation and virulence during citrus fruit infectiones
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.entidadIVIACentro de Protección Vegetal y Biotecnologíaes
dc.journal.titleInternational Journal of Food Microbiologyes
dc.subject.agrisQ02 Food processing and preservationes
dc.subject.agrisJ10 Handling, transport, storage and protection of agricultural productses
dc.subject.agrovocTranscription factorses
dc.subject.agrovocPenicillium digitatumes

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Atribución-NoComercial-SinDerivadas 3.0 España
Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 España