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dc.contributor.authorSerra, Pedro
dc.contributor.authorBertolini, Edson
dc.contributor.authorMartínez, M. Carmen
dc.contributor.authorCambra, Mariano
dc.contributor.authorFlores, Ricardo
dc.date.accessioned2020-10-28T12:41:29Z
dc.date.available2020-10-28T12:41:29Z
dc.date.issued2017es
dc.identifier.citationSerra, P., Bertolini, E., Martínez, M. C., Cambra, M., & Flores, R. (2017). Interference between variants of peach latent mosaic viroid reveals novel features of its fitness landscape: implications for detection. Scientific reports, 7, 42825.es
dc.identifier.issn2045-2322 (online)
dc.identifier.urihttp://hdl.handle.net/20.500.11939/6714
dc.description.abstractNatural populations of peach latent mosaic viroid (PLMVd) are complex mixtures of variants. During routine testing, TaqMan rtRT-PCR and RNA gel-blot hybridization produced discordant results with some PLMVd isolates. Analysis of the corresponding populations showed that they were exclusively composed of variants (of class II) with a structural domain different from that of the reference and many other variants (of class I) targeted by the TaqMan rtRT-PCR probe. Bioassays in peach revealed that a representative PLMVd variant of class II replicated without symptoms, generated a progeny with low nucleotide diversity, and, intriguingly, outcompeted a representative symptomatic variant of class I when co-inoculated in equimolecular amounts. A number of informative positions associated with the higher fitness of variants of class II have been identified, and novel sets of primers and probes for universal or specific TaqMan rtRT-PCR detection of PLMVd variants have been designed and tested.es
dc.language.isoenes
dc.publisherSpringer Naturees
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectVirus-host interactionses
dc.titleInterference between variants of peach latent mosaic viroid reveals novel features of its fitness landscape: implications for detectiones
dc.typearticlees
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.entidadIVIACentro de Protección Vegetal y Biotecnologíaes
dc.identifier.doi10.1038/srep42825es
dc.identifier.urlhttps://www.nature.com/articles/srep42825es
dc.journal.issueNumber7es
dc.journal.titleScientific Reportses
dc.page.final42825es
dc.page.initial42825es
dc.rights.accessRightsopenAccesses
dc.source.typeelectronicoes
dc.subject.agrisH20 Plant diseaseses
dc.subject.agrovocVirologyes
dc.type.hasVersionpublishedVersiones


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