Genetic diversity reflects geographical origin of Ralstonia solanacearum strains isolated from plant and water sources in Spain
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Caruso, Paola; Biosca, Elena G.; Bertolini, Edson; Marco-Noales, Ester; Gorris, María T.; Licciardello, Concetta; López, María M.Date
2017Cita bibliográfica
Caruso, P., Biosca, E. G., Bertolini, E., Marco-Noales, E., Gorris, M. T., Licciardello, C., & López, M. M. (2017). Genetic diversity reflects geographical origin of Ralstonia solanacearum strains isolated from plant and water sources in Spain. International Microbiology, 20(4), 155-164.Abstract
The characterization and intraspecific diversity of a collection of 45 Ralstonia solanacearum strains isolated
in Spain from different sources and geographical origins is reported. To test the influence of the site and the host on strain
diversity, phenotypic and genotypic analysis were performed by a polyphasic approach. Biochemical and metabolic profiles
were compared. Serological relationship was evaluated by Indirect-ELISA using polyclonal and monoclonal antibodies.
For genotypic analysis, hrpB and egl DNA sequence analysis, repetitive sequences (rep-PCR), amplified fragment length
polymorphism (AFLP) profiles and macrorestriction with XbaI followed by pulsed field gel electrophoresis (PFGE) were
performed.
The biochemical and metabolic characterization, serological tests, rep-PCR typing and phylogenetic analysis showed that all
analysed strains belonged to phylotype II sequevar 1 and shared homogeneous profiles. However, interesting differences among
strains were found by AFLP and macrorestriction with XbaI followed by PFGE techniques, some profiles being related to the
geographical origin of the strains. Diversity results obtained offer new insights into the biogeography of this quarantine organism
and its possible sources and reservoirs in Spain and Mediterranean countries.