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dc.contributor.authorGaravello, Miguel
dc.contributor.authorCuenca, José
dc.contributor.authorDreissig, Steven
dc.contributor.authorFuchs, Jörg
dc.contributor.authorHouben, Andreas
dc.contributor.authorAleza, Pablo
dc.date.accessioned2020-02-21T11:51:31Z
dc.date.available2020-02-21T11:51:31Z
dc.date.issued2019
dc.identifier.citationGaravello, M., Cuenca, J., Dreissig, S., Fuchs, J., Houben, A., & Aleza, P. (2019). Assessing Ploidy Level Analysis and Single Pollen Genotyping of Diploid and Euploid Citrus Genotypes by Fluorescence-Activated Cell Sorting and Whole-Genome Amplification. Frontiers in Plant Science, 10.
dc.identifier.issn1664-462X
dc.identifier.urihttp://hdl.handle.net/20.500.11939/6305
dc.description.abstractFlow cytometry is widely used to determine genome size and ploidy level in plants. This technique, when coupled with fluorescence-activated cell sorting (FACS), whole genome amplification and genotyping (WGA), opens up new opportunities for genetic studies of individualized nuclei. This strategy was used to analyze the genetic composition of single pollen nuclei of different citrus species. The flow cytometry and microscope observations allowed us to differentiate the populations of pollen nuclei present in the diploid and euploid genotypes analyzed, showing that citrus has binuclear pollen. We have identified in the "CSO" tangor an additional nuclei population composed by the vegetative plus generative nuclei. Genotyping of this nuclei population revealed that vegetative and generative nuclei show the same genetic configuration. In addition, we have demonstrated the presence of unreduced gametes in the diploid genotype "Mexican lime." Genomic amplification is a robust method for haploid nuclei genotyping with several molecular markers, whereas in diploid nuclei using heterozygous markers showed a bias towards one of the two alleles, limiting the use of this tool in this type of nuclei. We further discuss the importance and applications of single pollen genotyping in citrus genetic studies.
dc.language.isoen
dc.titleAssessing Ploidy Level Analysis and Single Pollen Genotyping of Diploid and Euploid Citrus Genotypes by Fluorescence-Activated Cell Sorting and Whole-Genome Amplification
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.entidadIVIACentro de Citricultura y Producción Vegetal
dc.identifier.doi10.3389/fpls.2019.01174
dc.identifier.urlhttps://www.frontiersin.org/articles/10.3389/fpls.2019.01174/full
dc.journal.titleFrontiers In Plant Science
dc.journal.volumeNumber10
dc.page.initial1174
dc.rights.accessRightsopenAccess
dc.source.typeelectronico
dc.type.hasVersionpublishedVersion


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