Show simple item record

dc.contributor.authorSánchez-Torres, Paloma
dc.contributor.authorVilanova, Laura
dc.contributor.authorBallester, Ana Rosa
dc.contributor.authorLópez-Pérez, Mario
dc.contributor.authorTeixidó, Neus
dc.contributor.authorVinas, Inmaculada
dc.contributor.authorUsall, Josep
dc.contributor.authorGonzalez-Candelas, Luis
dc.contributor.authorTorres, Rosario
dc.date.accessioned2019-05-15T10:37:43Z
dc.date.available2019-05-15T10:37:43Z
dc.date.issued2018es
dc.identifier.citationSánchez-Torres, P.; Vilanova, L.; Rosa Ballester, A.; López-Pérez, M.; Teixido, N.; Vinas, I.; Usall, J.; Gonzalez-Candelas, L.; Torres, R. (2018). Unravelling the contribution of the penicillium expansum PeSte12 transcription factor to virulence during apple fruit infection. Food Microbiology, 69, 123-135.es
dc.identifier.urihttp://hdl.handle.net/20.500.11939/6241
dc.description.abstractBlue mould disease caused by Penicillium expansum infection is one of the most important diseases of pome fruit accounting for important economic losses. In the present study, the PeSte12 transcription factor gene was identified, and deletant mutants were produced by gene replacement. Knockout mutants showed a significant decrease of virulence during apple fruit infection. Virulence was affected by the maturity stage of the fruit (immature, mature and over-mature), and disease severity was notably reduced when the apples were stored at 0 degrees C. The Delta PeSte12 mutants resulted defective in asexual reproduction, producing less conidia, but this characteristic did not correlate with differences in microscopic morphology. In addition, the DPeSte12 mutants produced higher quantity of hydrogen peroxide than the wild type strain. Gene expression analysis revealed that PeSte12 was induced over time during apple infection compared to axenic growth, particularly from 2 dpi, reinforcing its role in virulence. Analysis of transcriptional abundance of several genes in Delta PeSte12 mutants showed that in most of the evaluated genes, PeSte12 seemed to act as a negative regulator during axenic growth, as most of them exhibited an increasing expression pattern along the time period evaluated. The highest expression values corresponded to detoxification, ATPase activity, protein folding and basic metabolism. Gene expression analysis during apple infection showed that 3 out of 9 analysed genes were up regulated; thus, PeSte12 seemed to exert a positive control to particular type of aldolase. These results demonstrate the PeSte12 transcription factor could play an important role in P. expansum's virulence and asexual reproduction. (C) 2017 Elsevier Ltd. All rights reserved.es
dc.language.isoenes
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.titleUnravelling the contribution of the Penicillium expansum PeSte12 transcription factor to virulence during apple fruit infectiones
dc.typearticlees
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.entidadIVIACentro de Protección Vegetal y Biotecnologíaes
dc.identifier.doi10.1016/j.fm.2017.08.005es
dc.journal.titleFood Microbiologyes
dc.journal.volumeNumber69es
dc.page.final135es
dc.page.initial123es
dc.source.typeelectronicoes
dc.type.hasVersionacceptedVersion


Files in this item

This item appears in the following Collection(s)

Show simple item record

Atribución-NoComercial-SinDerivadas 3.0 España
Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 España