Show simple item record

dc.contributor.authorLopez-Soriano, Pablo
dc.contributor.authorNoguera, Patricia
dc.contributor.authorGorris, María T.
dc.contributor.authorPuchades, Rosa
dc.contributor.authorMaquieira, Angel
dc.contributor.authorMarco-Noales, Ester
dc.contributor.authorLópez, María M.
dc.date.accessioned2018-05-09T16:30:59Z
dc.date.available2018-05-09T16:30:59Z
dc.date.issued2017
dc.identifier.citationLopez-Soriano, P., Noguera, P., Gorris, M. T., Puchades, R., Maquieira, A., Marco-Noales, E., Lopez, M. M. (2017). Lateral flow immunoassay for on-site detection of xanthomonas arboricola pv. pruni in symptomatic field samples. Plos One, 12(4), e0176201.
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/20.500.11939/6062
dc.description.abstractXanthomonas arboricola pv. pruni is a quarantine pathogen and the causal agent of the bacterial spot disease of stone fruits and almond, a major threat to Prunus species. Rapid and specific detection methods are essential to improve disease management, and therefore a prototype of a lateral flow immunoassay (LFIA) was designed for the detection of X. arboricola pv. pruni in symptomatic field samples. It was developed by producing polyclonal antibodies which were then combined with carbon nanoparticles and assembled on nitrocellulose strips. The specificity of the LFIA was tested against 87 X. arboricola pv. pruni strains from different countries worldwide, 47 strains of other Xanthomonas species and 14 strains representing other bacterial genera. All X. arboricola pv. pruni strains were detected and cross-reactions were observed only with four strains of X. arboricola pv. corylina, a hazelnut pathogen that does not share habitat with X. arboricola pv. pruni. The sensitivity of the LFIA was assessed with suspensions from pure cultures of three X. arboricola pv. pruni strains and with spiked leaf extracts prepared from four hosts inoculated with this pathogen (almond, apricot, Japanese plum and peach). The limit of detection observed with both pure cultures and spiked samples was 10(4) CFU ml(-1). To demonstrate the accuracy of the test, 205 samples naturally infected with X. arboricola pv. pruni and 113 samples collected from healthy plants of several different Prunus species were analyzed with the LFIA. Results were compared with those obtained by plate isolation and real time PCR and a high correlation was found among techniques. Therefore, we propose this LFIA as a screening tool that allows a rapid and reliable diagnosis of X. arboricola pv. pruni in symptomatic plants.
dc.language.isoen
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.titleLateral flow immunoassay for on-site detection of Xanthomonas arboricola pv. pruni in symptomatic field samples
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1371/journal.pone.0176201
dc.identifier.url
dc.journal.abbreviatedTitle
dc.journal.issueNumber4
dc.journal.titlePlos One
dc.journal.volumeNumber12
dc.page.finale0176201
dc.page.initial
dc.source.typeelectronico
dc.type.hasVersionpublishedVersion


Files in this item

This item appears in the following Collection(s)

Show simple item record

Atribución-NoComercial-SinDerivadas 3.0 España
Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 España