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dc.contributor.authorMonzó, César
dc.contributor.authorUrbaneja, Alberto
dc.contributor.authorXimenez-Embun, Miguel
dc.contributor.authorGarcia-Fernandez, Julia
dc.contributor.authorGarcía, José L.
dc.contributor.authorCastanera, Pedro
dc.date.accessioned2017-06-01T10:12:50Z
dc.date.available2017-06-01T10:12:50Z
dc.date.issued2012
dc.identifier.citationMonzó, C., Urbaneja, A., Ximénez-Embún, M., García-Fernández, J., García, J. L., & Castañera, P. (2012). Selection of Ceratitis capitata (Diptera: Tephritidae) specific recombinant monoclonal phage display antibodies for prey detection analysis. PloS one, 7(12), e51440.
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/20.500.11939/5685
dc.description.abstractSeveral recombinant antibodies against the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), one of the most important pests in agriculture worldwide, were selected for the first time from a commercial phage display library of human scFv antibodies. The specificity and sensitivity of the selected recombinant antibodies were compared with that of a rabbit polyclonal serum raised in parallel using a wide range of arthropod species as controls. The selected recombinant monoclonal antibodies had a similar or greater specificity when compared with classical monoclonal antibodies. The selected recombinant antibodies were successfully used to detect the target antigen in the gut of predators and the scFv antibodies were sequenced and compared. These results demonstrate the potential for recombinant scFv antibodies to be used as an alternative to the classical monoclonal antibodies or even molecular probes in the post-mortem analysis studies of generalist predators. Citation: Monzo C, Urbaneja A, Ximenez-Embun M, Garcia-Fernandez J, Garcia JL, et al. (2012) Selection of Ceratitis capitata (Diptera: Tephritidae) Specific Recombinant Monoclonal Phage Display Antibodies for Prey Detection Analysis. PLoS ONE 7(12): e51440. doi:10.1371/journal.pone.0051440
dc.language.isoen
dc.titleSelection of Ceratitis capitata (Diptera: Tephritidae) Specific Recombinant Monoclonal Phage Display Antibodies for Prey Detection Analysis
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormDEC 14 2012
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1371/journal.pone.0051440
dc.journal.issueNumber12
dc.journal.titlePlos One
dc.journal.volumeNumber7
dc.page.finale51440
dc.page.initiale51440
dc.rights.accessRightsopenAccess
dc.source.typeImpreso
dc.type.hasVersionpublishedVersion


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