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dc.contributor.authorAmbrós, Silvia
dc.contributor.authorEl-Mohtar, Choaa
dc.contributor.authorRuiz-Ruiz, Susana
dc.contributor.authorPena, Leandro
dc.contributor.authorGuerri, José
dc.contributor.authorDawson, William O.
dc.contributor.authorMoreno, Pedro
dc.date.accessioned2017-06-01T10:12:45Z
dc.date.available2017-06-01T10:12:45Z
dc.date.issued2011
dc.identifier.citationAmbros, S., El-Mohtar, Choaa, Ruiz-Ruiz, S., Pena, L., Guerri, J., Dawson, William O., Moreno, P. (2011). Agroinoculation of Citrus tristeza virus Causes Systemic Infection and Symptoms in the Presumed Nonhost Nicotiana benthamiana. Molecular Plant-Microbe Interactions, 24(10), 1119-1131.
dc.identifier.issn0894-0282
dc.identifier.urihttp://hdl.handle.net/20.500.11939/5654
dc.description.abstractCitrus tristeza virus (CTV) naturally infects only some citrus species and relatives and within these it only invades phloem tissues. Failure to agroinfect citrus plants and the lack of an experimental herbaceous host hindered development of a workable genetic system. A full-genome cDNA of CTV isolate T36 was cloned in binary plasmids and was used to agroinfiltrate Nicotiana benthamiana leaves, with or without coinfiltration with plasmids expressing different silencing-suppressor proteins. A time course analysis in agroinfiltrated leaves indicated that CTV accumulates and moves cell-to-cell for at least three weeks postinoculation (wpi), and then, it moves systemically and infects the upper leaves with symptom expression. Silencing suppressors expedited systemic infection and often increased infectivity. In systemically infected Nicotiana benthamiana plants, CTV invaded first the phloem, but after 7 wpi, it was also found in other tissues and reached a high viral titer in upper leaves, thus allowing efficient transmission to citrus by stem-slash inoculation. Infected citrus plants showed the symptoms, virion morphology, and phloem restriction characteristic of the wild T36 isolate. Therefore, agroinfiltration of Nicotiana benthamiana provided the first experimental herbaceous host for CTV and an easy and efficient genetic system for this closterovirus.
dc.language.isoen
dc.titleAgroinoculation of Citrus tristeza virus Causes Systemic Infection and Symptoms in the Presumed Nonhost Nicotiana benthamiana
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormOCT 2011
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1094/MPMI-05-11-0110
dc.journal.abbreviatedTitleMol.Plant-Microbe Interact.
dc.journal.issueNumber10
dc.journal.titleMolecular Plant-Microbe Interactions
dc.journal.volumeNumber24
dc.page.final1131
dc.page.initial1119
dc.rights.accessRightsopenAccess
dc.source.typeImpreso
dc.type.hasVersionpublishedVersion


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