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dc.contributor.authorMestre, P. F.
dc.contributor.authorAsins, María J.
dc.contributor.authorPina, José A.
dc.contributor.authorCarbonell, Emilio A.
dc.contributor.authorNavarro, Luis
dc.identifier.citationMestre, PF, Asins, M.J., Pina, JA, Carbonell, EA, Navarro, L. (1997). Molecular markers flanking citrus tristeza virus resistance gene from Poncirus trifoliata (L), Raf. Theoretical and Applied Genetics, 94(3-4), 458-464.
dc.description.abstractTwo segregating populations for citrus tristeza virus (CTV) resistance derived from Poncirus trifoliata var 'Flying Dragon' by self-pollination and pollination to Citrus medica L. var ethrog 'Arizona' were inoculated with a common CTV isolate. The presence of virus was checked by the Double Antibody Sandwich Enzyme-Linked Assay and Direct Tissue Blot Inmunoassay at 3, 6, and 12 months after inoculation. Seven RAPDs were found linked to the CTV resistance gene by bulked segregant analysis. The closest linked RAPDs were cloned to obtain linked codominant RFLPs and to increase the precision of the tic distance estimation. The CTV resistance gene seems to be located between cW18 and cK16. Differences in genetic distances among progenies are large and can be explained by genome-wide reduction in the recombination of progeny derived from male versus female gametes.
dc.titleMolecular markers flanking citrus tristeza virus resistance gene from Poncirus trifoliata (L) Raf
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.entidadIVIACentro de Citricultura y Producción Vegetal
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.journal.titleTheoretical and Applied Genetics

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