Show simple item record

dc.contributor.authorMaliogka, Varvara
dc.contributor.authorOlmos, Antonio
dc.contributor.authorPappi, Polyxeni G.
dc.contributor.authorLotos, Leonidas
dc.contributor.authorEfthimiou, Konstantinos
dc.contributor.authorGrammatikaki, Garyfalia
dc.contributor.authorCandresse, Thierry
dc.contributor.authorKatis, Nikolaos
dc.contributor.authorAvgelis, Apostolos D.
dc.date.accessioned2017-06-01T10:12:33Z
dc.date.available2017-06-01T10:12:33Z
dc.date.issued2015
dc.identifier.citationMaliogka, V. I., Olmos, A., Pappi, P. G., Lotos, L., Efthimiou, K., Grammatikaki, G. et al. (2015). A novel grapevine badnavirus is associated with the Roditis leaf discoloration disease. Virus research, 203, 47-55.
dc.identifier.issn0168-1702
dc.identifier.urihttp://hdl.handle.net/20.500.11939/5558
dc.description.abstractRoditis leaf discoloration (RLD), a graft-transmissible disease of grapevine, was first reported in Greece in the 1980s. Even though various native grapevine viruses were identified in the affected vines, the etiology of the disease remained unknown. In the present study, we used an NGS platform for sequencing siRNAs from a twenty-year old Roditis vine showing typical RLD symptoms. Analysis of the NGS data revealed the presence of various known grapevine viruses and viroids as well as a hitherto uncharacterized DNA virus. The circular genome of the new virus was fully reassembled. It is 6988 nts long and includes 4 open reading frames (ORFs). ORF1, ORF2 and ORF4 code for proteins with unknown functions while ORF3 encodes a polyprotein with motifs related to the replication, encapsidation and movement of the virus. Phylogenetic analysis classified the novel virus within the genus Badnavirus, with closest relationship to Fig badnavirus 1. Further studies showed that the new badnavirus is closely related with the RLD disease and the provisional name grapevine Roditis leaf discoloration-associated virus (GRLDaV) is proposed. Our findings extend the number of DNA viruses identified in grapevine, further drawing attention to the potential importance of this virus group on grapevine pathology.
dc.language.isoen
dc.titleA novel grapevine badnavirus is associated with the Roditis leaf discoloration diseaseen
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormMAY 4 2015
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1016/j.virusres.2015.03.003
dc.identifier.urlhttps://www.sciencedirect.com/science/article/abs/pii/S0168170215001215?via%3Dihub
dc.journal.abbreviatedTitleVirus Res.
dc.journal.titleVirus research
dc.journal.volumeNumber203
dc.page.final55
dc.page.initial47
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record