• Castellano
  • English
  • Valenciá
Página de inicio de ReDivia
Página de la Generalitat ValenciáPágina de IVIA
View Item 
  •   ReDivia Home
  • 1.- Investigación
  • 1.1.- Artículos de revista académica
  • View Item
  •   ReDivia Home
  • 1.- Investigación
  • 1.1.- Artículos de revista académica
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Selective enrichment improves isolation, serological and molecular detection of plant pathogenic bacteria

Export
untranslatedRefworks
URI
http://hdl.handle.net/20.500.11939/5515
DOI
10.1007/978-94-009-0043-1_25
Derechos de acceso
openAccess
Metadata
Show full item record
Author
López, María M.; Gorris, María T.; Llop, Pablo; Cubero, Jaime; Vicedo, Begonya; Cambra, Mariano
Date
1997
Cita bibliográfica
Lopez, M.M., Gorris, M. T., Llop, P., Cubero, J., Vicedo, B., Cambra, M. (1997). Selective enrichment improves isolation, serological and molecular detection of plant pathogenic bacteria. Diagnosis and Identification of Plant Pathogens, 11, 117-121.
Abstract
The detection of low numbers of plant pathogenic bacteria can be improved by using a combination of enrichment in selective media and specific monoclonal antibodies (mab) or specific primers. In this way, the enrichment increases the number of the target bacteria and the specific probes can accurately identify them in spite of some increase of the total bacterial population in the samples. The enrichment step must be optimised for each bacterium by defining the medium, time, temperature and incubation conditions in order to obtain high numbers of the target bacterium that will easily detected by the specific probes in a second step. Such procedures have been applied to the detection of Agrobacterium tumefaciens causal agent of crown gall, and to the detection of Erwinia carotovora subsp. atroseptica casual agent of potato black leg and E. amylovora casual agent of fire blight.
Collections
  • 1.1.- Artículos de revista académica

Browse

All of ReDiviaCommunities & CollectionsBy Issue DateAuthorsTitlesSubjetcsCategoriesIVIA CentersThis CollectionBy Issue DateAuthorsTitlesSubjetcsCategoriesIVIA Centers

My Account

LoginRegister

Statistics

View Usage Statistics

Of interest

IVIA Open Access PolicyIntellectual property and copyrightAutoarchiveFrequently Asked Questions

Indexers

Recolectauntranslated

El contenido de este sitio está bajo una licencia Creative Commons - No comercial - Sin Obra Derivada (by-nc-nd), salvo que se indique lo contrario.