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dc.contributor.authorLopes, S. A.
dc.contributor.authorBertolini, Edson
dc.contributor.authorFrare, G. F.
dc.contributor.authorMartins, E. C.
dc.contributor.authorWulff, N. A.
dc.contributor.authorTeixeira, D. C.
dc.contributor.authorFernandes, N. G.
dc.contributor.authorCambra, Mariano
dc.date.accessioned2017-06-01T10:12:28Z
dc.date.available2017-06-01T10:12:28Z
dc.date.issued2009
dc.identifier.citationLopes, S. A., Bertolini, E., Frare, G. F., Martins, E. C., Wulff, N. A., Teixeira, D. C., Fernandes, N. G., Cambra, M. (2009). Graft Transmission Efficiencies and Multiplication of 'Candidatus Liberibacter americanus' and 'Ca. Liberibacter asiaticus' in Citrus Plants. Phytopathology, 99(3), 301-306.
dc.identifier.issn0031-949X
dc.identifier.urihttp://hdl.handle.net/20.500.11939/5507
dc.description.abstractIn Brazil 'Candidatus Liberibacter asiaticus' and 'Ca. L. americanus' cause huanglongbing (also known as greening), the most destructive citrus disease. A shift in pathogen prevalence was observed over time, with a disproportional increase in 'Ca. L. asiaticus' occurrence. Graft transmission experiments were used for a comparative study of both species using budsticks from symptomatic branches of field-affected trees as inoculum. The plants were inoculated with 'Ca. L. asiaticus' or 'Ca. L. americanus' alone, or simultaneously with both species. Symptom manifestation and conventional and quantitative real-time polymerase chain reaction were used for plant evaluations. 'Ca. L. americanus' was detected mainly in symptomatic plants and 'Ca. L. asiaticus' was detected in symptomatic plants as well as in infected plants prior to symptom manifestation. Transmission percentages varied from 54.7 to 88.0% for 'Ca. L. asiaticus' and 10.0 to 45.2% for 'Ca. L. americanus' in two experiments. In co-inoculated plants, 12.9% contained 'Ca. L. americanus' only, 40.3% contained 'Ca. L. asiaticus' only, and 19.3% contained both species. Average bacterial titers for 'Ca. L. asiaticus' and 'Ca. L. americanus', in log cells per gram of leaf midrib, were 6.42 and 4.87 for the experimental plants and 6.67 and 5.74 for the field trees used as the source of inoculum. The higher bacterial populations of the 'Ca. L. asiaticus'-infected plants provided an explanation for the disproportional increase in field prevalence of this species over time, based on the greater likelihood for pathogen transmission by the insect vector.
dc.language.isoen
dc.titleGraft Transmission Efficiencies and Multiplication of 'Candidatus Liberibacter americanus' and 'Ca. Liberibacter asiaticus' in Citrus Plants
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormMAR 2009
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1094/PHYTO-99-3-0301
dc.journal.abbreviatedTitlePhytopathology
dc.journal.issueNumber3
dc.journal.titlePhytopathology
dc.journal.volumeNumber99
dc.page.final306
dc.page.initial301
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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