• Castellano
  • English
  • Valenciá
Página de inicio de ReDivia
Página de la Generalitat ValenciáPágina de IVIA
View Item 
  •   ReDivia Home
  • 1.- Investigación
  • 1.1.- Artículos de revista académica
  • View Item
  •   ReDivia Home
  • 1.- Investigación
  • 1.1.- Artículos de revista académica
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Tracking Agrobacterium strains by a RAPD system to identify single colonies from plant tumours

Export
untranslatedRefworks
URI
http://hdl.handle.net/20.500.11939/5501
DOI
10.1023/A:1023547300243
Derechos de acceso
openAccess
Metadata
Show full item record
Author
Llop, Pablo; Lastra, Beatriz; Marsal, H.; Murillo, Jesús; López, María M.
Date
2003
Cita bibliográfica
Llop, P., Lastra, B., Marsal, H., Murillo, J. & Lopez, M. M. (2003). Tracking Agrobacterium strains by a RAPD system to identify single colonies from plant tumours. European Journal of Plant Pathology, 109(4), 381-389.
Abstract
A molecular typing system for Agrobacterium strains based on the polymerase chain reaction-random amplified polymorphic DNA (PCR-RAPD) procedure was developed. It employs one to four different 10-mer primers and the results are highly reproducible. The band patterns obtained with the four primers for each of the 39 Agrobacterium strains analysed were different enough to differentiate the strains from each other. Strains with similar chromosomal background but different plasmid content, e. g. strains C58 and A281, gave the same band pattern with all the primers. Ten host plants were inoculated with eight Agrobacterium strains and the isolates obtained from the resulting tumours were analysed using the RAPD system developed here. The procedure allowed rapid identification of isolates recovered from tumours by comparison of their band patterns with band patterns of strains used as inoculum. The procedure also discriminated the various strains analysed. Purified bacterial cell suspensions, used for RAPD analyses, produced the same results as purified DNA, and greatly simplified the procedure. This system can be applied for rapid screening of Agrobacterium-like colonies isolated from plant tumours for epidemiological and genetic diversity studies.
Collections
  • 1.1.- Artículos de revista académica

Browse

All of ReDiviaCommunities & CollectionsBy Issue DateAuthorsTitlesSubjetcsCategoriesIVIA CentersThis CollectionBy Issue DateAuthorsTitlesSubjetcsCategoriesIVIA Centers

My Account

LoginRegister

Statistics

View Usage Statistics

Of interest

IVIA Open Access PolicyIntellectual property and copyrightAutoarchiveFrequently Asked Questions

Indexers

Recolectauntranslated

El contenido de este sitio está bajo una licencia Creative Commons - No comercial - Sin Obra Derivada (by-nc-nd), salvo que se indique lo contrario.