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dc.contributor.authorGarcía-Mengual, Elena
dc.contributor.authorSilvestre, Miguel A.
dc.contributor.authorSalvador, I.
dc.contributor.authorCebrián-Serrano, Alberto
dc.contributor.authorGarcia-Rosello, E.
dc.date.accessioned2017-06-01T10:11:58Z
dc.date.available2017-06-01T10:11:58Z
dc.date.issued2015
dc.identifier.citationGarcia-Mengual, E., Silvestre, M.A., Salvador, I., Cebrian-Serrano, A., Garcia-Rosello, E. (2015). Male pronucleus formation after ICSI: effect of oocyte cysteine or sperm Triton X-100 treatments. Czech Journal of Animal Science, 60(6), 241-249.
dc.identifier.issn1212-1819
dc.identifier.urihttp://hdl.handle.net/20.500.11939/5252
dc.description.abstractIn pigs, intracytoplasmic sperm injection (ICSI) efficiency is still poor. The inadequate decondensation of the sperm chromatin, its transformation into the male pronucleus (MPN) together with the subsequent inability to activate the oocyte, seem to be the main causes of the low ICSI efficiency. In order to improve the MPN formation we took two different approaches. On the one hand, the in vitro culture (IVC) medium postICSI was supplemented with 1.71mM cysteine (CYS). Alternatively, the sperm membrane was digested with Triton X-100 (TX) before ICSI, to improve the exposure of the sperm chromatin to the oocyte cytoplasm. After 6 h post-ICSI, the activation rate was significantly higher in TX group (70.0%) compared with CYS and control groups (42.2% and 48.9%, respectively; P < 0.05). However, no significant differences between the three groups were observed in terms of the number of pronuclei, 2PN (oocytes with 2 pronuclei and no visible sperm), and 1PN + sperm (oocytes with 1 pronucleus and one sperm head). At 22 h post-ICSI, the activation rates were similar in TX, CYS, and control groups (73.1, 78.9, and 75.7%, respectively). In addition, we did not observe significant differences between TX, CYS, and control groups for the number of pronuclei, 2PN (52.6, 56.7, and 50%, respectively) or 1PN + sperm (21.1, 33.3, and 32.1%, respectively). While no cleavage was observed in the CYS group, no significant differences in the cleavage rate were observed between control (21.3%) and TX (10.5%) groups. In summary, and under our conditions, neither CYS supplement, nor sperm TX pre-treatment were able to improve MPN formation at 6 and 22 h post-ICSI. However, the sperm TX pre-treatment improved oocyte activation at 6 h post-ICSI, although 22 h post-ICSI such a beneficial effect did not persist.
dc.language.isoen
dc.titleMale pronucleus formation after ICSI: effect of oocyte cysteine or sperm Triton X-100 treatments
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeForm2015
dc.entidadIVIACentro de Tecnología Animal
dc.identifier.doi10.17221/8237-CJAS
dc.journal.issueNumber6
dc.journal.titleCzech Journal of Animal Science
dc.journal.volumeNumber60
dc.page.final249
dc.page.initial241
dc.rights.accessRightsopenAccess
dc.source.typeImpreso
dc.type.hasVersionpublishedVersion


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