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dc.contributor.authorGalipienso, Luis
dc.contributor.authorVives, María C.
dc.contributor.authorMoreno, Pedro
dc.contributor.authorMilne, R. G.
dc.contributor.authorNavarro, Luis
dc.contributor.authorGuerri, José
dc.identifier.citationGalipienso, L., Vives, M.C., Moreno, P., Milne, R. G., Navarro, L., Guerri, J. (2001). Partial characterisation of citrus leaf blotch virus, a new virus from Nagami kumquat. Archives of Virology, 146(2), 357-368.
dc.description.abstractCitrus leaf blotch virus (CLBV) was purified from leaves of Nagami kumquat SRA-153 that showed bud union crease when propagated on Troyer citrange. Virions were filamentous particles (960 x 14 nm) containing a 42 kDa protein and a single-stranded RNA (ssRNA) of about 9,000 nt (Mr 3 x 10(6)). Infected tissue contained three species of double-stranded RNA (dsRNA) of Mr 6, 4.5 and 3.4 x 10(6). The nucleotide sequence of several complementary DNA (cDNA) clones showed significant similarities with replication-related proteins from plant filamentous viruses in several genera. A digoxigenin-labelled probe from one of these cDNA clones hybridised in Northern blots with ssRNA from virions and with the three dsRNA species, suggesting that the ssRNA is the genomic RNA of the virus, the largest dsRNA is its replicative form, and the two smaller dsRNAs probably replicative forms of 5' co-terminal subgenomic RNAs. CLBV was also detected in several citrus cultivars from Spain and Japan including Navelina sweet orange field trees propagated on Troyer citrange showing bud union crease; however, no virus could be detected in other citrus trees with similar symptoms. This indicates that CLBV is not restricted to kumquat SRA-153, but its involvement in causing the bud union disorder remains unclear.
dc.titlePartial characterisation of citrus leaf blotch virus, a new virus from Nagami kumquat
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.journal.titleArchives of Virology

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