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dc.contributor.authorFerrer, Rosa M.
dc.contributor.authorLuis-Arteaga, Marisol
dc.contributor.authorGuerri, José
dc.contributor.authorMoreno, Pedro
dc.contributor.authorRubio, Luis
dc.identifier.citationFerrer, R. M., L.-Arteaga, M., Guerri, J., Moreno, P., Rubio, L. (2007). Detection and identification of species of the genus Fabavirus by RT-PCR with a single pair of primers. Journal of virological methods, 144(1-2), 156-160.
dc.description.abstractThe genus Fabavirus includes three species: Broad bean wilt virus 1 (BBWV-1), BBWV-2 and Lamium mild mosaic virus (LMMV), but a new candidate species, Gentian mosaic virus (GeMV), has been proposed. Analysis of the complete nucleotide sequences of fabaviruses was used to design a pair of conserved primers for specific detection of members of this genus. These primers encompassed the 5'-terminal non-translatable region (NTR), whose size for BBWV-1, BBWV-2 and GeMV was different. RT-PCR, with this pair of primers, is a rapid and sensitive procedure for diagnosis of fabavirus infections, that also allows identification of distinct species involved in single or mixed infections, based on the size of the amplification products. Moreover, it might allow future discovery of potential new species of this genus. (c) 2007 Elsevier B.V. All rights reserved.
dc.titleDetection and identification of species of the genus Fabavirus by RT-PCR with a single pair of primers
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes 2007
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.journal.titleJournal of virological methods

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