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dc.contributor.authorFagoaga, Carmen
dc.contributor.authorLopez, C.
dc.contributor.authorHermoso-De-Mendoza, Alfonso
dc.contributor.authorMoreno, Pedro
dc.contributor.authorNavarro, Luis
dc.contributor.authorFlores, R.
dc.contributor.authorPena, Leandro
dc.date.accessioned2017-06-01T10:11:50Z
dc.date.available2017-06-01T10:11:50Z
dc.date.issued2006
dc.identifier.citationFagoaga, C., Lopez, C., Hermoso-de-Mendoza, A., Moreno, P., Navarro, L., Flores, R., Pena, L. (2006). Post-transcriptional gene silencing of the p23 silencing suppressor of Citrus tristeza virus confers resistance to the virus in transgenic Mexican lime. Plant Molecular Biology, 60(2), 153-165.
dc.identifier.issn0167-4412
dc.identifier.urihttp://hdl.handle.net/20.500.11939/5171
dc.description.abstractPreviously, we have shown that most Mexican limes (Citrus aurantifolia (Christ.) Swing.) expressing the p23 gene of Citrus tristeza virus (CTV) exhibit aberrations resembling viral leaf symptoms. Here we report that five independent transgenic lines having normal phenotype displayed characteristics typical of post-transcriptional gene silencing (PTGS): multiple copies of the transgene, low levels of the corresponding mRNA, methylation of the silenced transgene, and accumulation of p23-specific small interfering RNAs (siRNAs). When graft- or aphid-inoculated with CTV, some propagations of these silenced lines were immune: they neither expressed symptoms nor accumulated virions and viral RNA as estimated by DAS-ELISA and Northern blot hybridization, respectively. Other propagations were moderately resistant because they became infected later and showed attenuated symptoms compared to controls. The susceptible propagations, in addition to symptom expression and elevated virus titer, accumulated p23-specific siRNAs at levels significantly higher than immune or non-inoculated propagations, and showed transgene demethylation. This variable response among clonal transformants indicates that factors other than the genetic background of the transgenic plants play a key role in PTGS-mediated resistance.
dc.language.isoen
dc.titlePost-transcriptional gene silencing of the p23 silencing suppressor of Citrus tristeza virus confers resistance to the virus in transgenic Mexican lime
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormJAN 2006
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1007/s11103-005-3129-7
dc.journal.abbreviatedTitlePlant Mol.Biol.
dc.journal.issueNumber2
dc.journal.titlePlant Molecular Biology
dc.journal.volumeNumber60
dc.page.final165
dc.page.initial153
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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