• Castellano
  • English
  • Valenciá
Página de inicio de ReDivia
Página de la Generalitat ValenciáPágina de IVIA
View Item 
  •   ReDivia Home
  • 1.- Investigación
  • 1.1.- Artículos de revista académica
  • View Item
  •   ReDivia Home
  • 1.- Investigación
  • 1.1.- Artículos de revista académica
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Development of a CiFT Co-expression System for Functional Analysis of Genes in Citrus Flowers and Fruit

View/Open
Open 2009_Endo_Development.pdf (459.1Kb)
Export
untranslatedRefworks
URI
http://hdl.handle.net/20.500.11939/5161
http://hdl.handle.net/20.500.11939/5161
DOI
10.2503/jjshs1.78.74
Derechos de acceso
openAccess
Metadata
Show full item record
Author
Endo, Tomoko; Shimada, Takehiko; Fujii, Hiroshi; Nishikawa, Fumie; Sugiyama, Aiko; Nakano, Michiharu; Shimizu, Tokurou; Kobayashi, Yasushi; Araki, Takashi; Pena, Leandro; Omura, Mitsuo
Date
2009
Cita bibliográfica
Endo, Tomoko, Shimada, Takehiko, Fujii, Hiroshi, Nishikawa, Fumie, Sugiyama, Aiko, Nakano, Michiharu, Shimizu, Tokurou, Kobayashi, Yasushi, Araki, Takashi, Pena, L., Omura, Mitsuo (2009). Development of a CiFT Co-expression System for Functional Analysis of Genes in Citrus Flowers and Fruit. Journal of the Japanese Society for Horticultural Science, 78(1), 74-83.
Abstract
We have developed a CiFT co-expression system for the rapid evaluation of transgenic citrus flowers and fruit. In order to co-express a target gene and the CiFT gene, a CiFT co-expression vector was constructed by inserting the fragment containing a chimeric target gene into the binary vector harboring P35S::CiFT. This system was applied to metabolically engineer aroma components in trifoliate orange (Poncirus trifoliata L. Raf). In order to reduce the limonene level, a gene encoding limonene synthase (CitMTSE1) from satsuma mandarin (Citrus unshiu Marc.) was introduced into trifolliate orange in an anti-sense orientation with the CiFT co-expression vector. Transgenic plants flowered extremely early, and began to produce normal fruit within 2 years of Agrobacterium infection. Transcripts of transgenes were accumulated in reproductive tissues of transgenic plants, and endogenous transcripts for limonene synthase were reduced. The ratio of limonene to other monoterpenes was significantly decreased in flowers and fruit of transgenic plants. These results clearly indicated that the CiFT co-expression system is a useful tool for the functional analysis of genes in citrus flowers and fruit. Such analyses are important for several applications, including the metabolic engineering of aroma components in citrus.
Collections
  • 1.1.- Artículos de revista académica

Browse

All of ReDiviaCommunities & CollectionsBy Issue DateAuthorsTitlesSubjetcsCategoriesIVIA CentersThis CollectionBy Issue DateAuthorsTitlesSubjetcsCategoriesIVIA Centers

My Account

LoginRegister

Statistics

View Usage Statistics

Of interest

IVIA Open Access PolicyIntellectual property and copyrightAutoarchiveFrequently Asked Questions

Indexers

Recolectauntranslated

El contenido de este sitio está bajo una licencia Creative Commons - No comercial - Sin Obra Derivada (by-nc-nd), salvo que se indique lo contrario.