Assignment of SNP Allelic Configuration in Polyploids Using Competitive Allele-Specific PCR: Application to Citrus Triploid Progenies

Abstract

Estimation of allele copy numbers for molecular markers has long been considered a challenge for polyploid species, while this process is essential for most genetic research. With the increasing availability and whole genome coverage of SNP markers, it is important to implement a versatile SNP genotyping method to efficiently assign allelic configuration in polyploids. This work demonstrates the usefulness of the KASPar method, based on competitive allele-specific PCR, for the assignment of SNP allelic configuration in an efficient, accurate, simple, and cost effective way and it has been successfully applied in genotyping citrus triploids.