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A broad exploration of a transgenic population of citrus: stability of gene expression and phenotype

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URI
http://hdl.handle.net/20.500.11939/5013
DOI
10.1007/s001220051338
Derechos de acceso
openAccess
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Author
Cervera, Magdalena; Pina, José A.; Juárez, José; Navarro, Luis; Pena, Leandro
Date
2000
Cita bibliográfica
Cervera, M., Pina, JA, Juarez, J., Navarro, L., Pena, L. (2000). A broad exploration of a transgenic population of citrus: stability of gene expression and phenotype. Theoretical and Applied Genetics, 100(5), 670-677.
Abstract
A collection of 70 transgenic citrus plants for the uidA and nptII genes have been maintained under screenhouse conditions over a period of 4-5 years. A detailed scanning of the plants allowed us to detect four phenotypic off-type plants and a large variation of transgene integration and expression patterns among the population. Off-type plants were analysed and characterised as nucellar tetraploids, probably originating from tetraploid starting tissues rather than from somaclonal variation events. Transgene integration and expression analyses revealed that: (I) a significant negative correlation was found between copy number and GUS activity; (2) rearrangements of the T-DNA inserts did not imply low expression levels; and (3) stability of integration and expression of the transgenes was confirmed for all the transformants grown under natural environmental conditions. These combined features validate transformation as a tool for the genetic improvement of citrus.
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