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dc.contributor.authorCaruso, Paola
dc.contributor.authorGorris, María T.
dc.contributor.authorCambra, Mariano
dc.contributor.authorPalomo, José L.
dc.contributor.authorCollar, Jesús
dc.contributor.authorLópez, María M.
dc.date.accessioned2017-06-01T10:11:29Z
dc.date.available2017-06-01T10:11:29Z
dc.date.issued2002
dc.identifier.citationCaruso, P., Gorris, M. T., Cambra, M., Palomo, J.L., Collar, J., Lopez, M.M. (2002). Enrichment double-antibody sandwich indirect enzyme-linked immunosorbent assay that uses a specific monoclonal antibody for sensitive detection of Ralstonia solanacearum in asymptomatic potato tubers. Applied and Environmental Microbiology, 68(7), 3634-3638.
dc.identifier.issn0099-2240
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4978
dc.description.abstractSensitive and specific routine detection of Ralstonia solanacearum in symptomless potato tubers was achieved by efficient enrichment followed by a reliable double-antibody sandwich indirect enzyme-linked immunosorbent assay based on the specific monoclonal antibody 8B-IVIA. This monoclonal antibody reacted with 168 typical R. solanacearum strains and did not recognize 174 other pathogenic or unidentified bacteria isolated from potato. The optimized protocol included an initial enrichment step consisting of shaking the samples in modified Wilbrink broth for 72 h at 29degreesC. This step enabled specific detection by the enzyme-linked immunosorbent assay of 1 to 10 CFU of R. solanacearum per ml of initial potato extract. Analysis of 233 commercial potato lots by this method provided results that coincided with the results of conventional methods.
dc.language.isoen
dc.titleEnrichment double-antibody sandwich indirect enzyme-linked immunosorbent assay that uses a specific monoclonal antibody for sensitive detection of Ralstonia solanacearum in asymptomatic potato tubers
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormJUL 2002
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1128/AEM.68.7.3634-3638.2002
dc.journal.abbreviatedTitleAppl.Environ.Microbiol.
dc.journal.issueNumber7
dc.journal.titleApplied and Environmental Microbiology
dc.journal.volumeNumber68
dc.page.final3638
dc.page.initial3634
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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