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dc.contributor.authorCapote, Nieves
dc.contributor.authorGorris, María T.
dc.contributor.authorMartínez, M. Carmen
dc.contributor.authorAsensio, M.
dc.contributor.authorOlmos, Antonio
dc.contributor.authorCambra, Mariano
dc.date.accessioned2017-06-01T10:11:26Z
dc.date.available2017-06-01T10:11:26Z
dc.date.issued2006
dc.identifier.citationCapote, N., Gorris, M. T., Martinez, M. C., Asensio, M., Olmos, A. & Cambra, M. (2006). Interference between D and M types of Plum pox virus in Japanese plum assessed by specific monoclonal antibodies and quantitative real-time reverse transcription-polymerase chain reaction. Phytopathology, 96(3), 320-325.
dc.identifier.issn0031-949X
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4959
dc.description.abstractThe dynamics of virus interference between two isolates of Plum pox virus (PPV) belonging to the main PPV types, D and M, were analyzed in Japanese plum (Prunus salicina) by challenge inoculations. To assess the consequences of a PPV-M infection on plum already infected with PPV-D, and vice versa (predominance of one of the strains, recombination, synergism, symptoms aggravation, and so on), 30 Japanese plum trees were graft inoculated with PPV-D or PPV-M isolates in quarantine conditions. One year postinoculation, in the event that the inoculated isolates were detected in the whole plant, a second challenge inoculation (PPV-M or PPV-D, respectively) was performed by grafting. The presence of PPV-D, PPV-M, or both was monitored for 7 years by double-antibody sandwich indirect enzyme-linked immunosorbent assay using specific monoclonal antibodies. Reverse transcription-polymerase chain reaction (RT-PCR) with D- and M-specific primers confirmed the serological typing. Real-time RT-PCR assays were performed using D- and M-specific fluorescent 3′ minor groove binder-DNA probes, which were able to detect and quantify PPV populations in the inoculated plants with greater precision. The presence of PPV-D in Japanese plum did not cross-protect the trees against PPV-M infection. In PPV-D-infected plants, the PPV-M strain used as challenge inoculum behaved differently depending on the plum cultivar assayed. In cv. Black Diamond, PPV-M invaded the plant progressively, displacing the previous PPV-D population; whereas, in cv. Sun Gold, both PPV isolates coexisted in the plant. In contrast, the PPV-D isolate used was unable to infect plants of both cultivars in which a PPV-M population already was established. After 7 years, no synergism was observed and no recombination event between PPV-D and PPV-M genomes was detected.
dc.language.isoen
dc.titleInterference between D and M types of Plum pox virus in Japanese plum assessed by specific monoclonal antibodies and quantitative real-time reverse transcription-polymerase chain reactionen
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormMAR 2006
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1094/PHYTO-96-0320
dc.identifier.urlhttps://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-96-0320
dc.journal.abbreviatedTitlePhytopathology
dc.journal.issueNumber3
dc.journal.titlePhytopathology
dc.journal.volumeNumber96
dc.page.final325
dc.page.initial320
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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