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dc.contributor.authorCambra, Miguel A.
dc.contributor.authorPalacio-Bielsa, Ana
dc.contributor.authorLópez, María M.
dc.date.accessioned2017-06-01T10:11:24Z
dc.date.available2017-06-01T10:11:24Z
dc.date.issued2004
dc.identifier.citationCambra, M. A., Palacio-Bielsa, A. & Lopez, M. M. (2004). Borage (Borago officinalis), is a new host of Pseudomonas cichorii in the Ebro Valley of Spain.. Plant Disease, 88(7), 769-769.
dc.identifier.issn0191-2917
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4942
dc.description.abstractBorage (Borago officinalis L.) is an important vegetable crop for consumption in the Ebro Valley of northeastern Spain. During the autumn and spring of the last 10 years, black necrotic lesions were observed in leaf petioles of white-flowered borage plants in greenhouses and seedbeds in Zaragoza, Spain. These lesions developed from the soil line and spread upward to the central vein of leaves. Severely infected leaves of mature and occasionally young plants become rotted. Longitudinal sections through the crown revealed severely necrotic cortical, vascular, and pith tissues. Isolations from infected roots, petioles, and leaves onto King's B medium yielded a gram-negative, rod-shaped bacterium with colonies that were fluorescent under UV light. Bacterial colonies were purified and characterized. The isolates were strictly aerobic, negative for levan production, soft rot of potato, and arginine dihydrolase activity, oxidase positive, and induced hypersensitive reaction in tobacco leaves (2). The bacteria were further identified as Pseudomonas cichorii by comparison of the 49 carbohydrate utilization profiles, API 50 CH (bioMérieux, Marcy l'Etoile, France), with the reference strain ICPPB 2827. Ten lettuce plants used as indicators and borage plants were inoculated by root and petiole injections of bacterial suspensions (108 CFU/ml) of the borage strains and the P. cichorii reference strain ICPPB 2827. Inoculated plants and controls were maintained in a growth chamber at 20 to 25°C with nearly 100% relative humidity. Symptoms of varnish spot, described in lettuce (1), and the black lesions initially observed in borage roots, petioles, and leaves were evident on all inoculated plants at 7 and 5 days after inoculation, respectively. No symptoms developed on control plants. A bacterium with identical characteristics to those described above was reisolated from the black lesions on inoculated plants. To our knowledge, this is the first report of P. cichorii as a pathogen of B. officinalis. Successful infection of borage plants was dependent on high humidity conditions, which is present because of the greater density of mature crops.
dc.language.isoen
dc.titleBorage (Borago officinalis) is a new host of Pseudomonas cichorii in the Ebro Valley of Spain.
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormJUL 2004
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1094/PDIS.2004.88.7.769A
dc.identifier.urlhttps://apsjournals.apsnet.org/doi/10.1094/PDIS.2004.88.7.769A
dc.journal.abbreviatedTitlePlant Dis.
dc.journal.issueNumber7
dc.journal.titlePlant Disease
dc.journal.volumeNumber88
dc.page.final769
dc.page.initial769
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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