Production and characterization of a monoclonal antibody specific to the M serotype of plum pox potyvirus
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AuthorBoscia, D.; Zeramdini, H.; Cambra, Mariano; Potere, O.; Gorris, María T.; Myrta, A.; DiTerlizzi, B.; Savino, V.
Cita bibliográficaBoscia, D., Zeramdini, H., Cambra, M., Potere, O., Gorris, M. T., Myrta, A., DiTerlizzi, B., Savino, V. (1997). Production and characterization of a monoclonal antibody specific to the M serotype of plum pox potyvirus. European Journal of Plant Pathology, 103(5), 477-480.
A monoclonal antibody to an Albanian isolate of plum pox potyvirus (PPV) was obtained (MAbAL), that specifically recognized strain M of this virus. The specificity of MAbAL, assessed by comparative ELISA on 130 PPV isolates of different geographical origin, 22 of which were also tested by comparative IC-PCR, gave consistent and highly reproducible results. MAbAL seems to be elicited by a stable surface determinant that makes it particularly suitable for successful use under a wide range of conditions. MAbAL is an useful addition to the panel of PPV-specific MAbs available to date.