Show simple item record

dc.contributor.authorAgüero, Jesús
dc.contributor.authorVives, María C.
dc.contributor.authorVelázquez, Karelia
dc.contributor.authorPina, José A.
dc.contributor.authorNavarro, Luis
dc.contributor.authorMoreno, Pedro
dc.contributor.authorGuerri, José
dc.date.accessioned2017-06-01T10:11:16Z
dc.date.available2017-06-01T10:11:16Z
dc.date.issued2014
dc.identifier.citationAguero, Jesus, del C. Vives, M., Velazquez, Karelia, A. Pina, J., Navarro, L., Moreno, P., Guerri, J. (2014). Effectiveness of gene silencing induced by viral vectors based on Citrus leaf blotch virus is different in Nicotiana benthamiana and citrus plants. Virology, 460, 154-164.
dc.identifier.issn0042-6822
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4888
dc.description.abstractVirus induced gene silencing (VIGS) is an effective technology for gene function analysis in plants. We assessed the VIGS effectiveness in Nicotiana benthamiana and citrus plants of different Citrus leaf blotch virus (CLBV)-based vectors, using insets of the phytoene desaturase (pds) gene. While in N. benthamiana the silencing phenotype was induced only by the construct carrying a 58-nt pds hairpin, in citrus plants all the constructs induced the silencing phenotype. Differences in the generation of secondary small interfering RNAs in both species are believed to be responsible for differential host-species effects. The ability of CLBV-based vectors to silence different endogenous citrus genes was further confirmed. Since CLBV-based vectors are known to be stable and induce VIGS in successive flushes for several months, these vectors provide an important genomic tool and it is expected that they will be useful to analyze gene function by reverse genetics in the long-lived citrus plants.
dc.language.isoen
dc.titleEffectiveness of gene silencing induced by viral vectors based on Citrus leaf blotch virus is different in Nicotiana benthamiana and citrus plants
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormJUL 2014
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1016/j.virol.2014.04.017
dc.journal.abbreviatedTitleVirology
dc.journal.titleVirology
dc.journal.volumeNumber460
dc.page.final164
dc.page.initial154
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record